Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation

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Garrigos, Murilo M. ; Oliveira, Fernando A. ; Nucci, Mariana P. ; Mamani, Javier B. ; Dias, Olivia F. M. ; Rego, Gabriel N. A. ; Junqueira, Mara S. ; Costa, Cicero J. S. ; Silva, Lucas R. R. ; Alves, Arielly H. ; Valle, Nicole M. E. ; Marti, Luciana ; Gamarra, Lionel F.

• Document type: Journal article
• Source: PHARMACEUTICS; v. 15, n. 3, p. 18-pg., 2023-03-01.

Abstract

Bone marrow transplantation is a treatment for a variety of hematological and non-hematological diseases. For the transplant success, it is mandatory to have a thriving engraftment of transplanted cells, which directly depends on their homing. The present study proposes an alternative method to evaluate the homing and engraftment of hematopoietic stem cells using bioluminescence imaging and inductively coupled plasma mass spectrometry (ICP-MS) associated with superparamagnetic iron oxide nanoparticles. We have identified an enriched population of hematopoietic stem cells in the bone marrow following the administration of Fluorouracil (5-FU). Lately, the cell labeling with nanoparticles displayed the greatest internalization status when treated with 30 mu g Fe/mL. The quantification by ICP-MS evaluate the stem cells homing by identifying 3.95 +/- 0.37 mu g Fe/mL in the control and 6.61 +/- 0.84 mu g Fe/mL in the bone marrow of transplanted animals. In addition, 2.14 +/- 0.66 mg Fe/g in the spleen of the control group and 2.17 +/- 0.59 mg Fe/g in the spleen of the experimental group was also measured. Moreover, the bioluminescence imaging provided the follow up on the hematopoietic stem cells behavior by monitoring their distribution by the bioluminescence signal. Lastly, the blood count enabled the monitoring of animal hematopoietic reconstitution and ensured the transplantation effectiveness. (AU)

• FAPESP's process: 16/21470-3 - Therapeutic action of mesenchymal stem cells from human bone marrow labeled with multimodal nanoparticles in diabetic rats subjected to stroke: study of cellular, molecular and functional mechanisms.
• Grantee: Lionel Fernel Gamarra Contreras
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/17868-4 - Therapeutic action of mesenchymal stem cells of the human bone marrow, marked with multimodal nanoparticles, in diabetic rats submitted to focal cerebral ischemia: study of cellular, molecular and functional mechanisms
• Grantee: Yolanda Oliveira Pinto
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/21070-3 - Evaluation of the therapeutic efficacy of physical activity-associated nanoparticle-labeled stem cells in the focal ischemia model
• Grantee: João Victor Matias Ferreira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation