Shotgun proteomics reveals changes in the pectoralis major muscle of broilers supplemented with passion fruit seed oil under cyclic heat stress conditions

Author(s)

Assuncao, Andrey Savio de Almeida ; Martins, Renata Aparecida ; Vieira, Jose Cavalcante Souza ; Rocha, Leone Campos ; Krenchinski, Fernanda Kaiser de Lima ; Buzalaf, Marilia Afonso Rabelo ; Sartori, Jose Roberto ; Padilha, Pedro de Magalhaes

• Document type: Journal article
• Source: Food Research International; v. 167, p. 8-pg., 2023-03-24.

Abstract

The aim of this study was to characterize the proteins differentially expressed in the pectoralis major muscle of broilers supplemented with passion fruit seed oil (PFSO) under cyclic heat stress conditions. Ninety one-day-old male chicks were housed in cages arranged in a climatic chamber, where they were kept under cyclic heat stress for eight hours a day from the beginning to the end of the experiment. The birds were divided into two experimental groups, one group supplemented with 0.9% PFSO and a control group (CON) without PFSO sup-plementation. At 36 days of age, 18 birds were slaughtered to collect muscle samples. From pools of breast fillet samples from each group, proteolytic cleavage of the protein extracts was performed, and later, the peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The 0.9% PFSO supplementation revealed the modulation of 57 proteins in the pectoralis major muscle of broilers exposed to cyclic heat stress. Among them, four proteins were upregulated, and 46 proteins were downregulated. In addition, seven proteins were expressed only in the CON group. These results suggest that PFSO may increase heat tolerance, with a possible reduction in oxidative stress, activation of neuroprotective mechanisms, protection against apoptosis, decrease in inflammatory responses, and regulation of energy metabolism. (AU)

• FAPESP's process: 19/02538-4 - Effect of mercury exposure frequency in riverside populations from the Amazon region: identification of possible biomarkers
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/09466-3 - Elucidation of physiological and functional aspects of proteins associated with mercury in pirarucu (Arapaima gigas)
• Grantee: Izabela da Cunha Bataglioli
• Support Opportunities: Scholarships in Brazil - Master