Evaluation of the release, stability and antioxidant activity of Brazilian red propolis extract encapsulated by spray-drying, spray-chilling and using the combination of both techniques

Author(s)

Sa, Samuel Henrique Gomes ; Mazzocato, Marcella Chalella ; Saliba, Ana Sofia M. C. ; Alencar, Severino M. ; Favaro-Trindade, Carmen Silvia

• Document type: Journal article
• Source: Food Research International; v. 164, p. 12-pg., 2023-01-03.

Abstract

Red propolis, originary from Northeast Brazil, has a unique composition and a great commercial interest. However, due to the presence of ethanol and its remarkable sensory characteristic, its application in food products is challenging. Thus, the aim of this work was to microencapsulate the red propolis extract by spray-drying, spray-chilling, and combining both techniques. The particles loaded with propolis extracts were char-acterised and evaluated according to the stability of phenolic compounds, flavonoids and formononetin, during 60 days of storage. In addition, the formononetin release was also monitored during the oral, gastric, and in-testinal phases in the in vitro digestion process. All produced particles presented matrix-type with size, distri-bution, shape, hygroscopicity, and dispersibility parameters that varied according to the carrier and encapsulation process applied. The techniques used to fabricate the particles efficiently obtained powdered propolis extract and protected the extract's bioactive compounds, total flavonoids and formononetin throughout the analysed period. The gastrointestinal release study presented distinctive releases in all phases (oral, gastric, and intestinal). The spray-dried particles, for example, released formononetin mainly in the oral stage. While the spray-chilled particles were primarily released in the intestinal phase, and coated particles were released gradually throughout the assay, reaching maximum relief in the intestinal phase. In conclusion, using micro-encapsulation techniques by spray-drying, spray-chilling, and their combination developed particles with different levels of protection during storage, releases and characteristics, which resulted in a range of possible applications in the food, feed, cosmetic, and pharmaceutical industries. (AU)

• FAPESP's process: 19/11248-0 - Production of Brazilian red propolis microcapsules for use as a natural preservative and development of functional foods: characterization, bioaccessibility and epithelial transport through Caco-2 cells
• Grantee: Severino Matias de Alencar
• Support Opportunities: Regular Research Grants