Antimicrobial behavior of titanium coating with chlorhexidine-doped thin film exposed to a biofilm supplemented with nicotine

Author(s)

Matos, Adaias Oliveira ; Rangel, Elidiane Cipriano ; Ricardo Barao, Valentim Adelino ; Gregory, Richard Lee

• Document type: Journal article
• Source: DENTAL MATERIALS JOURNAL; v. 42, n. 2, p. 8-pg., 2023-04-01.

Abstract

Because nicotine upregulates the growth of most oral bacteria, this in vitro study investigated the antimicrobial effect of chlorhexidine-doped thin film on commercially pure titanium against Fusobacterium nucleatum (F. nucleatum) biofilm supplemented with different concentrations of nicotine (0, 1, and 2 mg/mL). Biofilms were formed on a chlorhexidine-doped thin film on commercially-pure-titanium discs and compared to the control groups. Biofilm viability, total biofilm growth using a spectrophotometer, extracellular polysaccharide content, and pH variations were assessed as dependent variables. Data were submitted to ANOVA and Tukey honest significant difference tests (a=0.05). F. nucleatum biofilm growth was inhibited when exposed to chlorhexidine-doped thin film (p<0.05). Biofilm supplemented with nicotine did not impact the synthesis of EPS on the same type of treatment (p>0.05). The pH values were significantly increased with the increase of nicotine concentration (p<0.05). Chlorhexidine-doped thin film was effective in reducing F. nucleatum biofilm supplemented with nicotine. (AU)

• FAPESP's process: 17/21894-0 - Antimicrobial and biocompatibility potentials of hexamethyldisiloxane films incorporated with chlorhexidine on titanium surface using a co-infected in vitro reconstituted gingival oral epithelium model
• Grantee: Adaias Oliveira Matos
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/06117-5 - Development of hexamethyldisiloxane films incorporated with chlorhexidine onto titanium surface: antimicrobial potential and cytotoxicity
• Grantee: Adaias Oliveira Matos
• Support Opportunities: Scholarships in Brazil - Doctorate