Eco-Friendly Extraction of Green Coffee Oil for Industrial Applications: Its Antioxidant, Cytotoxic, Clonogenic, and Wound Healing Properties

Author(s)

Dias, Lucas D. ; Carbinatto, Fernanda Mansano ; Almeida, Isabelle da Silveira ; Blanco, Kate C. ; Marquele-Oliveira, Franciane ; Munari, Carla Carolina ; Bagnato, Vanderlei Salvador

• Document type: Journal article
• Source: FERMENTATION-BASEL; v. 9, n. 4, p. 13-pg., 2023-04-01.

Abstract

The development of natural oil-based cosmetic and pharmaceutical products presents great scientific and commercial interest. Herein, we aimed to extract green coffee oil from Arabic coffee by a sustainable cold-pressing method. Furthermore, this work aimed to characterize the obtained green coffee oil by Fourier-Transform Infrared (FT-IR) and ultraviolet-visible spectroscopies (UV-Vis), peroxide analysis, and fatty acids profile by gas chromatography-mass spectrometry (GC-MS). Moreover, the functional and biological properties of the obtained green coffee oil and a green-coffee oil-based commercial product (Energy up((R)), Dermociencia) were investigated. The green coffee oil presented linoleic and palmitic acids as the major fatty acids showing 44.8% and 35.4%, respectively. Moreover, this green coffee oil presented an antioxidant activity (EC50 7.64 mg/mL) and an absence of cytotoxic effects in keratinocyte cultures treated with up to 20 mg/mL. The obtained green coffee oil showed wound healing properties as well as clonogenic efficiency, a biological potential to induce the proliferative and migratory capacity of cells of human skin keratinocytes at 2.5 mg/mL. The samples presented high antioxidant activity and the absence of a cytotoxic effect, suggesting that green coffee oil is a promising natural product for cosmetic applications with wound healing properties. These results open new ways for the use of green coffee oil for the development of cosmetic and pharmaceutics natural-based products. (AU)

• FAPESP's process: 18/00106-7 - Photocatalyzed Reactions by using porphyrins and continuous flow conditions: Arylations of heterocycles and activated olefins.
• Grantee: Kleber Thiago de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/13569-8 - Study of the Mechanisms of Action in Photodynamic Therapy: From Photosensitizer to Practical Application
• Grantee: Lucas Danilo Dias
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants