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A restoration and extrapolation iterative method for band-limited fluorescence microscopy image

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Author(s):
Ponti, Moacir P. ; Mascarenhas, Nelson D. A. ; Suazo, Claudio A. T.
Total Authors: 3
Document type: Journal article
Source: 2009 XXII BRAZILIAN SYMPOSIUM ON COMPUTER GRAPHICS AND IMAGE PROCESSING (SIBGRAPI 2009); v. N/A, p. 3-pg., 2007-01-01.
Abstract

Optical microscopic images, especially with a nonconfocal microscope, are fundamentally limited because the optical transfer function (the Fourier transform of the point-spread function) is zero over a region of the spatial-frequency domain. Iterative algorithms were developed for the restoration and extrapolation of diffraction-limited imagery. In this paper we present the effectiveness of an iterative method based on the Richardson-Lucy algorithm for image restoration and a simultaneous modified version of Gerchberg-Papoulis method to extrapolate the spectrum and control the noise amplification. Good convergence stabilization results were achieved and also good numerical results were observed. (AU)

FAPESP's process: 02/07153-2 - Algorithms for tomographic reconstruction: optimization, restoration, quantification and clinical application
Grantee:Sergio Shiguemi Furuie
Support Opportunities: Research Projects - Thematic Grants