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Evaluation of the direct effects of radiation on the enamel, dentin and dental pulp of head and neck cancer patients

Grant number: 13/18402-8
Support type:Regular Research Grants
Duration: March 01, 2014 - February 29, 2016
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Alan Roger dos Santos Silva
Grantee:Alan Roger dos Santos Silva
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Assoc. researchers:Ana Carolina Prado Ribeiro e Silva ; Thaís Bianca Brandão

Abstract

Introduction: Radiation-related caries (RRC) is a severe and frequent toxicity that affects patients who underwent head and neck radiotherapy (HNR). RRC is considered to be a multifactorial disease of difficult management, which is mainly attributed to indirect toxicities of radiotherapy. However, the exact role of direct effects of radiation on the dental structure and its consequent participation in the onset and progression of RRC as well as to the reduced longevity of dental restorations has not been fully understood so far. Aims: The study will test the hypothesis that the direct effect of radiation has the ability to cause morphological changes in the microstructure of enamel, dentin, dentin-enamel junction (DEJ) and dental pulp of teeth extracted from patients who concluded HNR. Material and methods: 80 teeth divided into 4 groups will be studied. The test groups A (n=20) and B (n=20) will be composed by teeth affected by RRC and teeth of patients who concluded HNR but were not affected by RRC, respectively. The control groups C (n=20) and D (n=20) will be composed by carious and sound teeth, respectively from healthy patients. This study will be based on histological (light microscopy and polarized light microscopy) and ultrastructure (scanning electron microscopy) analysis to evaluate the microstructural preservation of enamel, dentin and DEJ. Additionally, immunohistochemical analysis will investigate the vascularization and innervation of the dental pulp and in situ zymography will be used to study the gelatinolytic activity of post-HNR dentin through confocal fluorescence microscope. Expected results: We expect to achieve new scientific evidence that will improve the understanding of the direct effects of radiation on the microstructure of teeth mineralized tissues and the dental pulp. Also, it is probable that the results of this study will clarify if the direct effects of HNR on teeth are relevant to the onset and progression of RRC. (AU)

Scientific publications (20)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GOUVEA VASCONCELLOS, ADRIELE FERREIRA; PALMIER, NATALIA RANGEL; RIBEIRO, ANA CAROLINA PRADO; NORMANDO, ANA GABRIELA COSTA; MORAIS-FARIA, KARINA; GOMES-SILVA, WAGNER; VECHIATO FILHO, ALJOMAR JOSE; DE GOES, MARIO FERNANDO; PAES LEME, ADRIANA FRANCO; BRANDAO, THAIS BIANCA; LOPES, MARCIO AJUDARTE; MARSH, PHILIP D.; SANTOS-SILVA, ALAN ROGER. Impact of Clustering Oral Symptoms in the Pathogenesis of Radiation Caries: A Systematic Review. Caries Research, v. 54, n. 2, p. 113-126, AUG 2020. Web of Science Citations: 1.
PAGLIONI, MARIANA DE PAULI; FARIA, KARINA MORAIS; PALMIER, NATALIA RANGEL; PRADO-RIBEIRO, ANA CAROLINA; BRITO E DIAS, REINALDO; DA GRACA PINTO, HENRIQUE; TREISTER, NATHANIEL SIMON; EPSTEIN, JOEL B.; MIGLIORATI, CESAR AUGUSTO; SANTOS-SILVA, ALAN ROGER; BRANDAO, THAIS BIANCA. Patterns of oral mucositis in advanced oral squamous cell carcinoma patients managed with prophylactic photobiomodulation therapy-insights for future protocol development. Lasers in Medical Science, v. 36, n. 2 JUL 2020. Web of Science Citations: 0.
PALMIER, NATALIA RANGEL; MIGLIORATI, CESAR AUGUSTO; PRADO-RIBEIRO, ANA CAROLINA; QUERIDO DE OLIVEIRA, MARIA CECILIA; VECHIATO FILHO, ALJOMAR JOSE; DE GOES, MARIO FERNANDO; BRANDAO, THAIS BIANCA; LOPES, MARCIO AJUDARTE; SANTOS-SILVA, ALAN ROGER. Radiation-related caries: current diagnostic, prognostic, and management paradigms. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 130, n. 1, p. 52-62, JUL 2020. Web of Science Citations: 0.
DE PAULI PAGLIONI, MARIANA; PALMIER, NATALIA RANGEL; PRADO-RIBEIRO, ANA CAROLINA; FREGNANI, EDUARDO RODRIGUES; GAVIAO, MARIA BEATRIZ DUARTE; BRANDAO, THAIS BIANCA; LOPES, MARCIO AJUDARTE; RIBEIRO, ANA PAULA DIAS; MIGLIORATI, CESAR AUGUSTO; SANTOS-SILVA, ALAN ROGER. The impact of radiation caries in the quality of life of head and neck cancer patients. SUPPORTIVE CARE IN CANCER, v. 28, n. 6, p. 2977-2984, JUN 2020. Web of Science Citations: 0.
FONSECA, JESSICA MONTENEGRO; MADRID TROCONIS, CRISTHIAN CAMILO; PALMIER, NATALIA RANGEL; GOMES-SILVA, WAGNER; PAGLIONI, MARIANA DE PAULI; DAMACENO ARAUJO, ANNA LUIZA; ARISTIZABAL ARBO-LEDA, LADY PAOLA; VECHIATO FILHO, ALJOMAR JOSE; GONZALEZ-ARRIAGADA, WILFREDO ALEJANDRO; DE GOES, MARIO FERNANDO; LOPES, MARCIO AJUDARTE; BRANDAO, THAIS BIANCA; VARGAS, PABLO AGUSTIN; PRADO RIBEIRO, ANA CAROLINA; SANTOS-SILVA, ALAN ROGER. The impact of head and neck radiotherapy on the dentine- enamel junction: a systematic review. MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL, v. 25, n. 1, p. E96-E105, JAN 2020. Web of Science Citations: 1.
DE PAULI PAGLIONI, MARIANA; ALVES, CAROLINA GUIMARAES BONFIM; FONTES, ELISA KAUARK; LOPES, MARCIO AJUDARTE; RIBEIRO, ANA CAROLINA PRADO; BRANDAO, THAIS BIANCA; MIGLIORATI, CESAR AUGUSTO; SANTOS-SILVA, ALAN ROGER. Is photobiomodulation therapy effective in reducing pain caused by toxicities related to head and neck cancer treatment? A systematic review. SUPPORTIVE CARE IN CANCER, v. 27, n. 11, p. 4043-4054, NOV 2019. Web of Science Citations: 0.
PALMIER, NATALIA RANGEL; MADRID, CRISTHIAN CAMILO; PAGLIONI, MARIANA DE PAULI; RIVERA, CESAR; LEBRE MARTINS, BEATRIZ NASCIMENTO F.; DAMACCNO ARAUJO, ANNA LUIZA; SALVAJOLI, JOAO VICTOR; DE GOES, MARIO FERNANDO; LOPES, MARCIO AJUDARTE; PRADO RIBEIRO, ANA CAROLINA; BRANDAO, THAIS BIANCA; SANTOS-SILVA, ALAN ROGER. Cracked tooth syndrome in irradiated patients with head and neck cancer. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 126, n. 4, p. 335+, OCT 2018. Web of Science Citations: 1.
PRADO-RIBEIRO, ANA-CAROLINA; SANTOS-SILVA, ALAN-ROGER; FARIA, KARINA-MORAIS; SILVA, WAGNER-GOMES; SIMONATO, LUCIANA-ESTEVAM; MOUTINHO, KARINA; BRANDAO, THAIS-BIANCA. Radiation-related superficial oral mucoceles: An under-recognized acute toxicity in head and neck cancer patients. MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL, v. 23, n. 5, p. E518-E523, SEP 1 2018. Web of Science Citations: 0.
BRANDAO, THAIS BIANCA; MORAIS-FARIA, KARINA; PRADO RIBEIRO, ANA CAROLINA; RIVERA, CESAR; SALVAJOLI, JOAO VICTOR; LOPES, MARCIO AJUDARTE; EPSTEIN, JOEL B.; ARANY, PRAVEEN R.; DE CASTRO, JR., GILBERTO; MIGLIORATI, CESAR AUGUSTO; SANTOS-SILVA, ALAN ROGER. Locally advanced oral squamous cell carcinoma patients treated with photobiomodulation for prevention of oral mucositis: retrospective outcomes and safety analyses. SUPPORTIVE CARE IN CANCER, v. 26, n. 7, p. 2417-2423, JUL 2018. Web of Science Citations: 8.
FARIA, KARINA MORAIS; PRADO RIBEIRO, ANA CAROLINA; BRANDAO, THAIS BIANCA; SILVA, WAGNER GOMES; LOPES, MARCIO AJUDARTE; PEREIRA, JULIANA; ALVES, MARCELO CORREA; GUEIROS, LUIZ ALCINO; SHINTAKU, WERNER HARUMITI; MIGLIORATI, CESAR AUGUSTO; SANTOS-SILVA, ALAN ROGER. Radiographic patterns of multiple myeloma in the jawbones of patients treated with intravenous bisphosphonates. JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, v. 149, n. 5, p. 382-391, MAY 2018. Web of Science Citations: 1.
FARIA, KARINA-MORAIS; BRANDAO, THAIS-BIANCA; SILVA, WAGNER-GOMES; PEREIRA, JULIANA; NEVES, FREDERICO-SAMPAIO; ALVES, MARCELO-CORREA; SHINTAKU, WERNER-HARUMITI; LOPES, MARCIO-AJUDARTE; RIBEIRO, ANA-CAROLINA-PRADO; MIGLIORATI, CESAR-AUGUSTO; SANTOS-SILVA, ALAN-ROGER. Panoramic and skull imaging may aid in the identification of multiple myeloma lesions. MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL, v. 23, n. 1, p. E38-E43, JAN 1 2018. Web of Science Citations: 1.
PALMIER, NATALIA RANGEL; PRADO RIBEIRO, ANA CAROLINA; FONSECA, JESSICA MONTENEGRO; SALVAJOLI, JOAO VICTOR; VARGAS, PABLO AGUSTIN; LOPES, MARCIO AJUDARTE; BRANDAO, THAIS BIANCA; SANTOS-SILVA, ALAN ROGER. Radiation-related caries assessment through the International Caries Detection and Assessment System and the Post-Radiation Dental Index. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 124, n. 6, p. 542-547, DEC 2017. Web of Science Citations: 3.
MADRID TROCONIS, CRISTHIAN CAMILO; SANTOS-SILVA, ALAN ROGER; BRANDAO, THAIS BIANCA; LOPES, MARCIO AJUDARTE; DE GOES, MARIO FERNANDO. Impact of head and neck radiotherapy on the mechanical behavior of composite resins and adhesive systems: A systematic review. Dental Materials, v. 33, n. 11, p. 1229-1243, NOV 2017. Web of Science Citations: 1.
GOMES-SILVA, WAGNER; PRADO RIBEIRO, ANA CAROLINA; DE CASTRO JUNIOR, GILBERTO; SALVAJOLI, JOAO VICTOR; PALMIER, NATALIA RANGEL; LOPES, MARCIO AJUDARTE; ROCHA, MARCELO MARQUES; DE GOES, MARIO FERNANDO; BRANDAO, THAIS BIANCA; SANTOS-SILVA, ALAN ROGER. Head and neck radiotherapy does not increase gelatinase (metalloproteinase-2 and-9) expression or activity in teeth irradiated in vivo. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 124, n. 2, p. 175-182, AUG 2017. Web of Science Citations: 2.
PRADO-RIBEIRO, ANA-CAROLINA; LUIZ, ANA-CLAUDIA; MONTEZUMA, MARCO-AURELIO; MAK, MILENA-PEREZ; SANTOS-SILVA, ALAN-ROGER; BRANDAO, THAIS-BIANCA. Orbital apex syndrome affecting head and neck cancer patients: A case series. MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL, v. 22, n. 3, p. E354-E358, MAY 1 2017. Web of Science Citations: 1.
PAGLIONI, MARIANA DE PAULI; SILVA, WAGNER GOMES; PEREIRA, JULIANA; MIGLIORATI, CESAR AUGUSTO; LOPES, MARCIO AJUDARTE; DE ALMEIDA, OSLEI PAES; GOES, MARIO FERNANDO; PRADO RIBEIRO, ANA CAROLINA; BRANDAO, THAIS BIANCA; SANTOS-SILVA, ALAN ROGER. Intravenous bisphosphonate therapy does not thicken cementum or change periodontal ligaments of cancer patients. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 123, n. 5, p. 591-599, MAY 2017. Web of Science Citations: 1.
GOMES-SILVA, WAGNER; PRADO-RIBEIRO, ANA CAROLINA; BRANDAO, THAIS BIANCA; MORAIS-FARIA, KARINA; DE CASTRO JUNIOR, GILBERTO; MAK, MILENA PEREZ; LOPES, MARCIO AJUDARTE; ROCHA, MARCELO MARQUES; SALO, TUULA; TJADERHANE, LEO; DE GOES, MARIO FERNANDO; SANTOS-SILVA, ALAN ROGER. Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries. Caries Research, v. 51, n. 3, p. 216-224, 2017. Web of Science Citations: 4.
MADRID, CRISTHIAN C.; PAGLIONI, MARIANA DE PAULI; LINE, SERGIO R.; VASCONCELOS, KARINA G.; BRANDAO, THAIS BIANCA; LOPES, MARCIO A.; SANTOS-SILVA, ALAN ROGER; DE GOES, MARIO FERNANDO. Structural Analysiis of Enamel in Teeth from Head-and-Neck Cancer Patients Who Underwent Radiotherapy. Caries Research, v. 51, n. 2, p. 119-128, 2017. Web of Science Citations: 8.
ALAN ROGER SANTOS-SILVA; PATRICIA DO SOCORRO QUEIROZ FEIO; PABLO AGUSTIN VARGAS; MARIA ELVIRA PIZZIGATTI CORREA; MARCIO AJUDARTE LOPES. cGVHD-Related Caries and Its Shared Features with Other 'Dry-Mouth'-Related Caries. Brazilian Dental Journal, v. 26, n. 4, p. 435-440, Ago. 2015.
FARIA, KARMA MORAIS; BRANDAO, THAIS BIANCA; PRADO RIBEIRO, ANA CAROLINA; GOUVEA VASCONCELLOS, ADRIELE FERREIRA; DE CARVALHO, ICARO THIAGO; DE ARRUDA, FERNANDO FREIRE; CASTRO JUNIOR, GILBERTO; GROSS, VANESSA CRISTINA; ALMEIDA, OSLEI PAES; LOPES, MARCIO AJUDARTE; SANTOS-SILVA, ALAN ROGER. Micromorphology of the Dental Pulp Is Highly Preserved in Cancer Patients Who Underwent Head and Neck Radiotherapy. JOURNAL OF ENDODONTICS, v. 40, n. 10, p. 1553-1559, OCT 2014. Web of Science Citations: 11.

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