Could Raman spectroscopy discriminate the biochemical alterations among prostate carcinoma and benign prostate tissues? An in vitro study

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Silveira, Landulfo, Jr. ; Leite, Katia Ramos M. ; Srougi, Miguel ; Silveira, Fabricio L. ; Pacheco, Marcos Tadeu T. ; Pasqualucci, Carlos A. ; Kollias, N ; Choi, B ; Zeng, H ; Kang, HW ; Knudsen, BE ; Wong, BJF ; Ilgner, JF ; Izdebski, K ; Suter, MJ ; Lam, S ; Brenner, M ; Gregory, KW ; Tearney, GJ ; Marcu, L ; Hirschberg, H ; Madsen, S ; MahadevanJansen, A ; Jansen, ED ; Mandelis, A

• Document type: Journal article
• Source: PHOTONIC THERAPEUTICS AND DIAGNOSTICS VIII, PTS 1 AND 2; v. 8207, p. 7-pg., 2012-01-01.

Abstract

This work evaluated possible alterations in the Raman spectra of human prostate tissues in vitro, by developing a Principal Components Analysis algorithm for discriminating prostate carcinoma (PrCa) and benign prostate tissue. Raman spectrum was measured using a Raman spectrometer (830 nm) with a fiber Raman probe. Most of the samples exhibited a very strong background fluorescence, which was decreased by photobleaching the fragment during 5 min. and the remaining fluorescence was removed by polynomial filtering. The spectra of prostate in the fingerprint region are dominated by bands of proteins (mainly collagen, elastin, actin). By comparing the spectra of PrCa with the benign prostate tissue, we found a very small difference, indicating similar biochemical constitution of benign and malignant prostate tissue. Principal Components Analysis and Mahalanobis distance could discriminate the spectra of both benign and PrCa tissues with reasonable sensitivity and specificity. (AU)

• FAPESP's process: 09/01788-5 - Dispersive Raman spectroscopy using optical fibers "Raman probe" applied to the diagnosis of neoplasia in the skin and prostate
• Grantee: Landulfo Silveira Junior
• Support Opportunities: Regular Research Grants