Improving the performance of a glucose biosensor using an ionic liquid for enzyme immobilization. On the chemical interaction between the biomolecule, the ionic liquid and the cross-linking agent

Author(s)

Galhardo, Kelly S. ; Torresi, Roberto M. ; Cordoba de Torresi, Susana I.

• Document type: Journal article
• Source: Electrochimica Acta; v. 73, p. 6-pg., 2012-07-01.

Abstract

The effect of the room temperature ionic liquid (1-butyl-2,3-dimethylimidazolium tetrafluoroborate ([BMMI][BF4])) on the immobilization of glucose oxidase (GOx) was studied. The electrochemical performance of biosensors prepared following different protocols indicated a beneficial effect of the ionic liquid on the analytical parameters. The chemical interaction between GOx, [BMMI][BF4] and glutaraldehyde was investigated using UV-visible spectroscopy (UV-vis) and circular dichroism (CD). Structural changes of the biomolecule were observed to depend on the method used for the immobilization. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/53199-3 - Molecular recognition and energy storage: fundamental studies concerning geometry, size and synthesis effects on the optimization of the chemical properties of electroactive materials
• Grantee: Roberto Manuel Torresi
• Support Opportunities: Research Projects - Thematic Grants