Third generation biosensors based on the interaction among reduced graphene oxide, ionic liquids and oxidase enzymes.

• Grant number: 11/15159-0
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): January 01, 2012
• Effective date (End): August 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal researcher: Susana Inés Córdoba de Torresi
• Grantee: Vinicius Romero Gonçales
• Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 09/53199-3 - Molecular recognition and energy storage: fundamental studies concerning geometry, size and synthesis effects on the optimization of the chemical properties of electroactive materials, AP.TEM

Abstract

The development of nanoscience has allowed the optimization of a series of parameters that make up an electrochemical biosensor, such as the interface between electrode and biological material, efficiency of the chemical signal transduction, response time and compatibility with biological matrices. Among the nanomaterials used for this purpose, the reduced graphene oxide appears as a candidate able to overcome the results obtained so far with conventional electrodes or even with biosensors formed by carbon nanotubes, presenting detections at lower potential with better analytical performances.Therefore, this project involves the construction of third generation biosensors by monitoring the direct electron transfer between reduced graphene oxide and oxidase enzymes. In order to optimize the performance of the biosensors, the project seeks to incorporate ionic liquids for the maintenance of electronic and chemical properties of reduced graphene oxide and also for improving the stability and catalytic activity of the enzymes.It is noteworthy that the use of reduced grapheme oxide in the third generation biosensors is a new scientific issue, lacking in understanding of phenomena involved in direct electron transfer between the carbon material and the biological component.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GORICALES, VINICIUS R.; COLOMBO, RAFAEL N. P.; MINADEO, MARCO A. O. S.; MATSUBARA, ELAINE Y.; MAURICIO ROSOLEN, J.; CORDOBA DE TORRESI, SUSANA I.. Three-dimensional graphene/carbon nanotubes hybrid composites for exploring interaction between glucose oxidase and carbon based electrodes. Journal of Electroanalytical Chemistry, v. 775, p. 235-242, AUG 15 2016. (11/15159-0, 11/08581-7, 12/22755-0)

BENEDETTI, TANIA M.; GONCALES, VINICIUS R.; CORDOBA DE TORRESI, SUSANA I.; TORRESI, ROBERTO M.. In search of an appropriate ionic liquid as electrolyte for macroporous manganese oxide film electrochemistry. Journal of Power Sources, v. 239, p. 1-8, OCT 1 2013. (08/57805-2, 11/15159-0, 09/53199-3, 12/02117-0)

COLOMBO, RAFAEL N. P.; PETRI, DENISE F. S.; CORDOBA DE TORRESI, SUSANA I.; GONCALES, VINICIUS R.. Porous Polymeric Templates on ITO Prepared by Breath Figure Method for Gold Electrodeposition. Electrochimica Acta, v. 158, p. 187-195, MAR 10 2015. (12/22755-0, 11/15159-0, 09/53199-3)

LIMACHI, DENIS G. B.; GONCALES, VINICIUS R.; CINTRA, ELAINE P.; CORDOBA DE TORRESI, SUSANA I.. Controlling hydrophilicity and electrocatalytic properties of metallic hexacyanoferrates/conducting polymers hybrids for the detection of H2O2. Electrochimica Acta, v. 110, n. SI, p. 459-464, NOV 1 2013. (10/17008-6, 11/15159-0, 09/53199-3)

JULIANA L. SANTOS; VINICIUS R. GONÇALES; ELAINE P. CINTRA; SUSANA I. CÓRDOBA DE TORRESI. SÍNTESE E CARACTERIZAÇÃO DE COMPÓSITOS ORGÂNICO-INORGÂNICOS MACROPOROSOS PARA O SENSORIAMENTO DE H2O2. Química Nova, v. 38, n. 2, p. 196-201, 2015-02-00. (10/08245-4, 11/15159-0, 09/53199-3)

ANTONIO, JADIELSON L. S.; LIRA, LUIZ M.; GONCALES, VINICIUS R.; CORDOBA DE TORRESI, SUSANA I.. Fully conducting hydro-sponges with electro-swelling properties tuned by synthetic parameters. Electrochimica Acta, v. 101, n. SI, p. 216-224, JUL 1 2013. (10/17511-0, 11/15159-0)

GONCALES, VINICIUS R.; GAITAN, MARTIN H.; BRAGATTO, ALANN DE O. P.; SOLER-ILLIA, GALO J. A. A.; BARALDO, LUIS M.; CORDOBA DE TORRESI, SUSANA I.. Correlation between pore size and reactivity of macro/mesoporous iron and copper hexacyanoferrates for H2O2 electrocatalysis. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 706, p. 48-54, OCT 1 2013. (11/15159-0, 10/08245-4)