Proteome analysis of spinal cord during the clinical course of monophasic experimental autoimmune encephalomyelitis

Author(s)

Farias, Alessandro S. ; Martins-de-Souza, Daniel ; Guimaraes, Leandro ; Pradella, Fernando ; Moraes, Adriel S. ; Facchini, Gustavo ; Novello, Jose Camillo ; Santos, Leonilda M. B.

• Document type: Journal article
• Source: PROTEOMICS; v. 12, n. 17, p. 7-pg., 2012-08-01.

Abstract

The induction of autoimmune encephalomyelitis (EAE) in Lewis rats results in a period of exacerbation followed by complete recovery. Therefore, this model is widely used for studying the evolution of multiple sclerosis. In the present investigation, differentially expressed proteins in the spinal cord of Lewis rats during the evolution of EAE were assessed using the combination of 2DE and MALDI-TOF MS. The majority of the differentially expressed proteins were identified during the acute phase of EAE, in relation to naive control animals. On the other hand, recovered rats presented a similar protein expression pattern in comparison with the naive ones. This observation can be explained, at least in part, by the intense catabolism existent in acute phase due to nervous tissue damage. In recovered rats, we have described the upregulation of proteins that are apparently involved in the recovery of damaged tissue, such as light and medium neurofilaments, glial fibrillary acidic protein, tubulins subunits, and quaking protein. These proteins are involved mainly in cell growth, myelination, and remyelination as well as in astrocyte and oligodendrocyte maturation. The present study has demonstrated that the inflammatory response, characterized by an increase of the proliferative response and infiltration of autoreactive T lymphocytes in the central nervous system, occurs simultaneously with neurodegeneration. (AU)

• FAPESP's process: 09/18227-6 - Effectors and immunoregulatory mechanisms in demyelinating diseases induced in human and experimental models
• Grantee: Leonilda Maria Barbosa dos Santos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/18728-5 - Study of migratory, effector and regulatory pattern of autoreactive t lymphocytes, previously transduced with GFP in experimental demyelinating diseases
• Grantee: Alessandro dos Santos Farias
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 09/15620-9 - Analysis of migratory pattern of effectors and regulatory cells during the clinical evolution of experimental autoimmune encephalomyelitis in Lewis rats and C57BL/6 mice (wild type and ifngamma KO)
• Grantee: Alessandro dos Santos Farias
• Support Opportunities: Scholarships in Brazil - Post-Doctoral