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Study of migratory, effector and regulatory pattern of autoreactive t lymphocytes, previously transduced with GFP in experimental demyelinating diseases

Grant number: 11/18728-5
Support type:Research Grants - Young Investigators Grants
Duration: March 01, 2012 - May 31, 2016
Field of knowledge:Biological Sciences - Immunology - Cellular Immunology
Principal researcher:Alessandro dos Santos Farias
Grantee:Alessandro dos Santos Farias
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):15/10107-2 - Characterization of thymus during the evolution of experimental encephalomyelitis, BP.PD
14/23628-8 - Study of the effect of G-CSF on the generation of tolerogenic dendritic cells in vitro and the action of these cells in vivo during the clinical evolution of the Experimental Autoimmune Neuritis, BP.IC
14/03622-5 - Study of the cytotoxic effect of the neuritogenic TCD4 lymphocytes over the Schwann Cells on the Experimental autoimmune neuritis, BP.IC
+ associated scholarships 14/03438-0 - Study of the CD4+CD8+ T lymphocytes during the evolution of the Experimental Autoimmune Neuritis, BP.IC
13/09085-9 - Study of the migratory, effector and regulatory pattern of plasmacytoid dendritic cells and B lymphocytes present in the central nervous system during the inflammatory process in experimental autoimmune encephalomyelitis in Lewis rats, BP.PD
13/10637-6 - Citotoxity activity of CD4+ encephalitogenic lymphocytes in Experimental Autoimmune Encephalomyelitis, BP.MS
12/21172-1 - Study of migratory, effector and regulatory patterns of cell present in the central nervous system during the inflammatory process of Experimental Autoimmune Encephalomyelitis, BP.DR
12/20935-1 - Study of the cytotoxic activity of CD4+ T lymphocytes in the Experimental Autoimmune Neuritis, BP.DR
12/05158-9 - citotoxic profile of CD4+ T cells during the clinical evolution of Experimental Autoimmune Encephalomyelitis, BP.IC
12/07133-3 - Analysis of Expression and Action of Cytotoxic Molecules in T Lymphocytes During the Evolution of Experimental Autoimmune Neuritis, BP.IC
12/01408-0 - Study of migratory, effector and regulatory pattern of autoreactive T lymphocytes, previously transduced with GFP in experimental demyelinating diseases, BP.JP - associated scholarships

Abstract

The experimental auto-immune encephalomyelitis (EAE) and experimental auto-immune neuritis are experimental models of autoimmune T cells mediated diseases, since these diseases can be adoptively transferred to naive animals by T CD4 lymphocytes transfer. The effector function of these lymphocytes, however, can be decreased by regulatory T lymphocytes. On the other hand, the auto-reactive T lymphocytes as well as the regulatory T lymphocytes are identified within the T CD4 lymphocytes population. Therefore, the labeling of effectors T lymphocytes opens up a possibility of understanding the migration of these lymphocytes to the nervous system (central or peripheral), as well as the interaction between the auto-reactive and regulatory cells that co-migrate to attenuate the inflammation on the nervous system. In this study, we will use the GFP retroviral transduction technique in auto-reactive T lymphocytes. This method allows a permanent labeling, allowing in vitro, ex vivo and in vivo analysis of auto-reactive T lymphocytes, by cytometry flow and/or confocal microscopy. Still, this technique allows the specific genes linked or not to GFP, serving as a tool for genetic introduction (knock in) in a determined cellular population. Besides that, this method of labeling will allow us the track of the auto-reactive T CD4 lymphocytes during its migration and the expression of cytotoxic as granzyme and perforin by these cells during the evolution of EAE and EAN. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VON GLEHN, FELIPE; JARIUS, SVEN; CAVALCANTI LIRA, RODRIGO PESSOA; ALVES FERREIRA, MARIA CAROLINA; RIBEIRO VON GLEHN, FADUA H.; COSTA E CASTRO, STELLA MARIS; BELTRAMINI, GUILHERME COCO; BERGO, FELIPE P. G.; FARIAS, ALESSANDRO S.; BRANDAO, CARLOS OTAVIO; WILDEMANN, BRIGITTE; DAMASCENO, BENITO P.; CENDES, FERNANDO; SANTOS, LEONILDA M. B.; YASUDA, CLARISSA LIN. Structural brain abnormalities are related to retinal nerve fiber layer thinning and disease duration in neuromyelitis optica spectrum disorders. MULTIPLE SCLEROSIS, v. 20, n. 9, p. 1189-1197, AUG 2014. Web of Science Citations: 27.
MORAES, ADRIEL S.; PAULA, ROSEMEIRE F. O.; PRADELLA, FERNANDO; SANTOS, MARIANA P. A.; OLIVEIRA, ELAINE C.; VON GLEHN, FELIPE; CAMILO, DANIELA S.; CERAGIOLI, HELDER; PETERLEVITZ, ALFREDO; BARANAUSKAS, VITOR; VOLPINI, WALKYRIA; FARIAS, ALESSANDRO S.; SANTOS, LEONILDA M. B. The Suppressive Effect of IL-27 on Encephalitogenic Th17 Cells Induced by Multiwalled Carbon Nanotubes Reduces the Severity of Experimental Autoimmune Encephalomyelitis. CNS Neuroscience & Therapeutics, v. 19, n. 9, p. 682-687, SEP 2013. Web of Science Citations: 12.
FARIAS, ALESSANDRO S.; SPAGNOL, GABRIELA S.; BORDEAUX-REGO, PEDRO; OLIVEIRA, CAMILA O. F.; FONTANA, ANA GABRIELA M.; DE PAULA, ROSEMEIRE F. O.; SANTOS, MARIANA P. A.; PRADELLA, FERNANDO; MORAES, ADRIEL S.; OLIVEIRA, ELAINE C.; LONGHINI, ANA LEDA F.; REZENDE, ALEXANDRE C. S.; VAISBERG, MAURO W.; SANTOS, LEONILDA M. B. Vitamin D-3 Induces IDO+ Tolerogenic DCs and Enhances Treg, Reducing the Severity of EAE. CNS Neuroscience & Therapeutics, v. 19, n. 4, p. 269-277, APR 2013. Web of Science Citations: 72.
FARIAS, ALESSANDRO S.; MARTINS-DE-SOUZA, DANIEL; GUIMARAES, LEANDRO; PRADELLA, FERNANDO; MORAES, ADRIEL S.; FACCHINI, GUSTAVO; NOVELLO, JOSE CAMILLO; SANTOS, LEONILDA M. B. Proteome analysis of spinal cord during the clinical course of monophasic experimental autoimmune encephalomyelitis. PROTEOMICS, v. 12, n. 17, SI, p. 2656-2662, AUG 2012. Web of Science Citations: 10.

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