Characterization and Shelf Life of beta-Carotene Loaded Solid Lipid Microparticles Produced With Stearic Acid and Sunflower Oil

Author(s)

de Lara Gomes, Graziela Veiga ; Borrin, Thais Ribeiro ; Cardoso, Lisandro Pavie ; Souto, Eliana ; de Pinho, Samantha Cristina

• Document type: Journal article
• Source: Brazilian Archives of Biology and Technology; v. 56, n. 4, p. 9-pg., 2013-07-01.

Abstract

Solid lipid microparticles were tested as microencapsulation systems for protecting beta-carotene from degradation. Blends of long-chain (C18) solid lipids (70% stearic acid) and sunflower oil (30%) were used to produce lipid microparticles encapsulating the carotenoid. Polysorbate 80 (4%) was employed to stabilize the stearic acid microparticles. The concentration of beta-carotene was monitored using spectrophotometry, the particle size distribution was measured by laser diffraction, the crystal structure was determined by wide angle X-ray diffraction (WAXD), and the thermal behaviour was characterized by differential scanning calorimetry (DSC) over a period of seven months. All of the systems had an average particle size smaller than 5 mu m. To avoid beta-carotene oxidation, alpha-tocopherol was added to the formulations and its action as an oxygen trap was crucial for the antioxidant effect. For stearic-acid microparticles with alpha-tocopherol, more than 90% of the initial amount of beta-carotene was preserved after seven months under refrigerated storage (7-10 degrees C) in the dark. Significant microstructural alterations were detected using WAXD and DSC only in the stearic acid microparticles without alpha-tocopherol. These results seemed promising and suggested that the blends of long-chain solid lipids and liquid lipids were suitable for the production of stable solid lipid microparticles. (AU)

• FAPESP's process: 09/02028-4 - Incorporation of beta-carotene in micro and nanoparticulate solid lipid systems: production, stability and microstructural characterization
• Grantee: Graziela Veiga de Lara Gomes
• Support Opportunities: Scholarships in Brazil - Master