Increase of the phytase production by Aspergillus japonicus and its biocatalyst potential on chicken feed treatment

Author(s)

Maller, Alexandre ; Vici, Ana Claudia ; Facchini, Fernanda Del Antonio ; da Silva, Tony Marcio ; Kamimura, Eliana Setsuko ; Rodrigues, Maria Isabel ; Jorge, Joao Atilio ; Terenzi, Hector Francisco ; Teixeira de Moraes Polizeli, Maria de Lourdes

• Document type: Journal article
• Source: JOURNAL OF BASIC MICROBIOLOGY; v. 54, p. 9-pg., 2014-07-01.

Abstract

Phytase hydrolyzes phytic acid from the plant components of animal feed, releasing inorganic phosphorus. The phytase production by Aspergillus japonicus was optimized using Plackett-Burman designs (PBD), composite central rotational designs (CCRD), and response surface methodology from standard Czapek medium. The enzyme was applied in broiler chicken and laying hen foods. Analysis from PBD showed that KH2PO2, MgSO4 center dot 7H(2)O, and yeast extract had significant influences on phytase secretion (p< 0.05). The best results from the CCRD experiments were obtained using (A) 0.040% KH2PO4, (B) 0.050% MgSO4 center dot 7H(2)O, and (C) 0.040% yeast extract, enhancing in 49-53Umg(-1) protein. The determination coefficient (R-2) was 0.92 and F-calc was 7.48 times greater than F-listed. Thus, the reduced coded model. Y (U mg(-1)) = 50.29 + 4.30A - 3.35(A)(2) - 4.80(B)(2) + 5.62C - 4.26(C)(2) was considered predictive and statistically significant (p< 0.05). The optimized culture medium increased the phytase yield in 250%. A. japonicus phytase released high levels of Pi from broiler chicken and laying hen food. A. japonicus is an excellent phytase producer in a culture medium using inexpensive components and agricultural wastes. Therefore, these results provide sound arguments for the formulation of a low cost culture medium for phytase production. (AU)