Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair

Author(s)

Biscola, Natalia Perussi ; Cartarozzi, Luciana Politti ; Ferreira Junior, Rui Seabra ; Barraviera, Benedito ; Rodrigues de Oliveira, Alexandre Leite

• Document type: Journal article
• Source: NEURAL PLASTICITY; v. 2016, p. 19-pg., 2016-01-01.

Abstract

Brachial plexus lesion results in loss of motor and sensory function, being more harmful in the neonate. Therefore, this study evaluated neuroprotection and regeneration after neonatal peripheral nerve coaptation with fibrin sealant. Thus, P2 neonatal Lewis rats were divided into three groups: AX: sciatic nerve axotomy (SNA) without treatment; AX+FS: SNA followed by end-to-end coaptation with fibrin sealant derived from snake venom; AX+CFS: SNA followed by end-to-end coaptation with commercial fibrin sealant. Results were analyzed 4, 8, and 12 weeks after lesion. Astrogliosis, microglial reaction, and synapse preservation were evaluated by immunohistochemistry. Neuronal survival, axonal regeneration, and ultrastructural changes at ventral spinal cord were also investigated. Sensory-motor recovery was behaviorally studied. Coaptation preserved synaptic covering on lesioned motoneurons and led to neuronal survival. Reactive gliosis and microglial reaction decreased in the same groups (AX+FS, AX+CFS) at 4 weeks. Regarding axonal regeneration, coaptation allowed recovery of greater number of myelinated fibers, with improved morphometric parameters. Preservation of inhibitory synaptic terminals was accompanied by significant improvement in the motor as well as in the nociceptive recovery. Overall, the present data suggest that acute repair of neonatal peripheral nerves with fibrin sealant results in neuroprotection and regeneration of motor and sensory axons. (AU)

• FAPESP's process: 14/06892-3 - Use of mesenchymal stem cells in the CNS/PNS interface: repair of proximal lesions
• Grantee: Alexandre Leite Rodrigues de Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 09/53846-9 - Recent acquisition of preparative high-performance liquid chromatograph for the purification and isolation of serine proteinases to produce fibrin sealant
• Grantee: Benedito Barraviera
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 12/08101-8 - In vivo imaging of nerve regeneration following ventral root replantation with fluo-stained fibrin sealant associated with mesenchymal stem cells
• Grantee: Rui Seabra Ferreira Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
• Grantee: Suely Vilela
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/23377-7 - Neuroprotective and regenerative effects of fibrin sealant derived from snake venom combined with mononuclear stem cells after neurorrhaphy of the sciatic nerve of neonatal rats
• Grantee: Natália Perussi Biscola
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)