Crystal structure of a putative exo-beta-1,3-galactanase from Bifidobacterium bifidum S17

Author(s)

Godoy, Andre S. ; de Lima, Mariana Z. T. ; Camilo, Cesar M. ; Polikarpov, Igor

• Document type: Journal article
• Source: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 72, p. 6-pg., 2016-04-01.

Abstract

Given the current interest in second-generation biofuels, carbohydrate-active enzymes have become the most important tool to overcome the structural recalcitrance of the plant cell wall. While some glycoside hydrolase families have been exhaustively described, others remain poorly characterized, especially with regard to structural information. The family 43 glycoside hydrolases are a diverse group of inverting enzymes; the available structure information on these enzymes is mainly from xylosidases and arabinofuranosidase. Currently, only one structure of an exo-beta-1,3-galactanase is available. Here, the production, crystallization and structure determination of a putative exo-beta-1,3-galactanase from Bifidobacterium bifidum S17 (BbGal43A) are described. BbGal43A was successfully produced and showed activity towards synthetic galactosides. BbGal43A was subsequently crystallized and data were collected to 1.4 angstrom resolution. The structure shows a single-domain molecule, differing from known homologues, and crystal contact analysis predicts the formation of a dimer in solution. Further biochemical studies are necessary to elucidate the differences between BbGal43A and its characterized homologues. (AU)

• FAPESP's process: 11/05712-3 - Structural and functional studies of beta-galactosidases from Xanthomonas campestris
• Grantee: Andre Schutzer de Godoy
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 09/52840-7 - Center of Biological and Industrial Processes for Biofuels - CeProBIO
• Grantee: Igor Polikarpov
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 11/20505-4 - Two important classes of glycosyl hydrolases: functional studies and structural analysis
• Grantee: Marco Antonio Seiki Kadowaki
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis
• Grantee: Igor Polikarpov
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 09/05328-9 - Expression, purification and cristalization studies of the ligand-binding domain of nuclear receptors PPAR-alpha e PPAR-gamma
• Grantee: Andre Schutzer de Godoy
• Support Opportunities: Scholarships in Brazil - Scientific Initiation