Repression activity of Tailless on h 1 and eve 1 pair-rule stripes

Author(s)

Andrioli, Luiz Paulo ; dos Santos, Wesley Silva ; Aguiar, Francisco dos Santos ; Digiampietri, Luciano Antonio

• Document type: Journal article
• Source: MECHANISMS OF DEVELOPMENT; v. 144, p. 7-pg., 2017-04-01.

Abstract

We investigated the hypothesis that several transcriptional repressors are necessary to set the boundaries of anterior pair-rule stripes in Drosophila. Specifically, we tested whether Tailless (Tll) is part of a repression mechanism that correctly sets the anterior boundaries of hairy 1 (h 1) and even-skipped 1 (eve 1) stripes. Single mutant tll embryos displayed subtle deviations from the normal positions of h 1 and eve 1 stripes. Moreover, we observed stronger stripe deviations in embryos lacking both Tll and Sloppy-paired 1 (Slp 1), a common repressor for anterior pair-rule stripes. Using h 1 and eve 1 reporter constructs in the genetic assays, we provided further evidence that interference with normal mechanisms of stripe expression is mediated by Tll repression. Indeed, Tll represses both h 1 and eve 1 reporter stripes when misexpressed. Investigating the expression of other anterior gap genes in different genetic backgrounds and in the misexpression assays strengthened Tll direct repression in the regulation of h 1 and eve 1. Our results are consistent with tll being a newly-identified component of a combinatorial network of repressor genes that control pair-rule stripe formation in the anterior blastoderm of Drosophila. (C) 2016 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 11/00096-2 - Investigating the segmentation cascade in the anterior regions of the embryo of Drosophila melanogaster and of the sciarid Trichosia pubescens
• Grantee: Luiz Paulo Moura Andrioli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/14318-5 - Gene interactions in the anterior region of the syncytial blastoderm of Drosophila melanogaster and of the sciarid fly Trichosia pubescens
• Grantee: Luiz Paulo Moura Andrioli
• Support Opportunities: Regular Research Grants