An In Vitro Model for the Study of the Macrophage Response Upon Trichophyton rubrum Challenge

Author(s)

Yamada Yoshikawa, Fabio Seiti ; Ferreira, Lucas Goncalves ; de Almeida, Fernando Goncalves ; de Almeida, Sandro Rogerio

• Document type: Journal article
• Source: Mycopathologia; v. 182, n. 1-2, p. 10-pg., 2017-02-01.

Abstract

Diversity in the macrophage models currently employed in immunology studies may lead to opposed results and interpretations. In this study, we aimed to analyze the suitability of J774 macrophage-like cells as a model for the interaction between the dermatophyte Trichophyton rubrum and macrophages. J774 cells were competent in fungal phagocytosis, but succumbed to hyphal growth. Nevertheless, they could also secrete IL-1 beta in response to the dermatophyte. On the opposite direction, inflammatory, thioglycollate-induced peritoneal macrophages did not succumb to fungal growth and showed no significant IL-1 beta production. The proteomic profiling of these cells uncovered vimentin and plastin-2 as proteins whose abundance was altered by the fungal interaction. Our study indicates that this cell line could be an interesting tool in the investigation of T. rubrum infection biology. (AU)

• FAPESP's process: 12/14684-6 - The role of the inflammasome in a murine model of infection with Trichophyton rubrum: activation pathways and in vivo relevance
• Grantee: Fábio Seiti Yamada Yoshikawa
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 10/07836-9 - Mechanism of cellular killing induced by Trychophyton rubrum after interaction with macrophages
• Grantee: Fábio Seiti Yamada Yoshikawa
• Support Opportunities: Scholarships in Brazil - Scientific Initiation