Bioelectrooxidation of Ethanol Using NAD-Dependent Alcohol Dehydrogenase on Oxidized Flexible Carbon Fiber Arrays

Author(s)

Pereira, Andressa R. ; de Souza, Joao C. P. ; Goncalves, Andressa D. ; Pagnoncelli, Kamila C. ; Crespilho, Frank N.

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 28, n. 9, p. 10-pg., 2017-09-01.

Abstract

Hierarchical 3-dimensional flexible carbon fiber (FCF) array is a candidate as flexible electrode for enzyme electrochemistry, with interesting properties for application in enzymatic biofuel cell and implantable biosensors. Here, we show the fabrication of oxidized FCF electrode (FCFO) based on chemical surface treatment, a wet oxidation method by using permanganate ions. Compared with pristine FCF, FCFO is rougher; the scratches become wider and deeper, with some defects in the basal planes and some cracked structures. X-ray photoelectron spectroscopy data suggests a correlation between the carbon structures and the appearance of chemical groups containing oxygen atoms. Finally, we show how the bioelectrocatalysis of ethanol by using FCFO arrays modified with NAD-dependent alcohol dehydrogenase (ADH) is positively influenced by the fibers treatment oxidation process. (AU)

• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/19908-2 - Bioelectrochemical study of the deglycosylated glucose oxidase enzyme for application in biofuel cells
• Grantee: Andressa Ribeiro Pereira
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/16672-3 - Development of High Performance Bioelectrodes for Application in Miniaturized Biofuel Cell
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants