| Full text | |
| Author(s): |
Scortecci, Jessica Fernandes
;
Balasco Serrao, Vitor Hugo
;
Cheleski, Juliana
;
Torini, Juliana Roberta
;
Romanello, Larissa
;
DeMarco, Ricardo
;
Pereira, Humberto D'Muniz
Total Authors: 7
|
| Document type: | Journal article |
| Source: | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1865, n. 11, p. 10-pg., 2017-11-01. |
| Abstract | |
The parasite Schistosoma mansoni possess all pathways for pyrimidine biosynthesis, whereby deaminases play an essential role in the thymidylate cycle, a crucial step to controlling the ratio between cytidine and uridine nucleotides. In this study, we heterologously expressed and purified the deoxycytidylate (dCMP) deaminase from S. mansoni to obtain structural, biochemical and kinetic information. Small-angle X-ray scattering of this enzyme showed that it is organized as a hexamer in solution. Isothermal titration calorimetry was used to determine the kinetic constants for dCMP-dUMP conversion and the role of dCTP and dTTP in enzymatic regulation. We evaluated the metals involved in activating the enzyme and show for the first time the dependence of correct folding on the interaction of two metals. This study provides information that may be useful for understanding the regulatory mechanisms involved in the metabolic pathways of S. mansoni. Thus, improving our understanding of the function of these essential pathways for parasite metabolism and showing for the first time the hitherto unknown deaminase function in this parasite. (AU) | |
| FAPESP's process: | 12/05532-8 - Structural and kinetic studies of adenosine kinase, hypoxanthine-guanine phosphoribosyltransferase, Adenilsuccinate Synthetase and Adenilsuccinato Lyase enzymes from Schistosoma mansoni |
| Grantee: | Larissa Romanello |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/14223-9 - Structural and kinetical studies of the enzymes involved in the purine metabolism in Schistosoma mansoni |
| Grantee: | Humberto D'Muniz Pereira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/15777-8 - Kinetic and structural studies of the Deoxycytidylate deaminase (DEOC) from Schistosoma mansoni |
| Grantee: | Jessica Fernandes Scortecci |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 12/23730-1 - Characterization of macromolecular interactions of proteins involved in the selenocysteine synthesis from Escherichia coli |
| Grantee: | Vitor Hugo Balasco Serrão |
| Support Opportunities: | Scholarships in Brazil - Doctorate |