Eluent Preparation for Hydrophilic Interaction Liquid Chromatography, Part 2: pH, Buffers, and Gradient Elution

Author(s)

Stoll, Dwight R. ; Seidl, Claudia

• Document type: Journal article
• Source: LC GC EUROPE; v. 31, n. 3, p. 5-pg., 2018-03-01.

Abstract

When preparing buffers for hydrophilic interaction chromatography (HILIC) separations, is the buffer concentration considered relative to the aqueous portion of the eluent only, or to the aqueous-organic mixture? (AU)

• FAPESP's process: 16/02941-5 - Development and optimization of two-dimensional liquid chromatography methodologies coupled with bioassays for discovery of enzyme inhibitors in natural product extracts
• Grantee: Cláudia Seidl
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor