| Full text | |
| Author(s): |
Mancini, Melina C.
;
Cardoso-Silva, Claudio B.
;
Sforca, Danilo A.
;
de Souza, Anete Pereira
Total Authors: 4
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PLANT SCIENCE; v. 9, p. 8-pg., 2018-03-28. |
| Abstract | |
Sugarcane exhibits a complex genome mainly due to its aneuploid nature and high ploidy level, and sequencing of its genome poses a great challenge. Closely related species with well-assembled and annotated genomes can be used to help assemble complex genomes. Here, a stable quantitative trait locus (QTL) related to sugar accumulation in sorghum was successfully transferred to the sugarcane genome. Gene sequences related to this QTL were identified in silico from sugarcane transcriptome data, and molecularmarkers based on these sequences were developed to select bacterial artificial chromosome (BAC) clones from the sugarcane variety SP80-3280. Sixty-eight BAC clones containing at least two gene sequences associated with the sorghum QTL were sequenced using Pacific Biosciences (PacBio) technology. Twenty BAC sequences were found to be related to the syntenic region, of which nine were sufficient to represent this region. The strategy we propose is called "targeted sequencing by gene synteny," which is a simpler approach to understanding the genome structure of complex genomic regions associated with traits of interest. (AU) | |
| FAPESP's process: | 17/05014-0 - Analysis of genes involved in a QTL for brix in Saccharum species |
| Grantee: | Melina Cristina Mancini |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 14/11482-9 - Evaluation of genomic synteny between sorghum (Sorghum bicolor) and sugarcane (Saccharum officinarum): comparision of genes involved in a QTL for Brix in sorghum to syntenic region in sugarcane |
| Grantee: | Melina Cristina Mancini |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 15/16399-5 - Balance between dosage and allelic expression to map the global gene expression profile in sugarcane |
| Grantee: | Cláudio Benício Cardoso da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/50119-6 - Identificação de cópias alélicas de locos que controlam caracteres de importância econômica e análise de sua variação entre cromossomos hom(e)ologas em cana-de-açúcar (Saccharum spp.) |
| Grantee: | Danilo Augusto Sforça |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 08/52197-4 - Genomic-assisted breeding of sugarcane: using molecular markers for understanding the genetic architecture of quantitative traits and to implement marker assisted selection |
| Grantee: | Anete Pereira de Souza |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |