Draft genome sequence of a bla(CMY-2)/IncI1-harbouring Escherichia coli D:ST457 isolated from coastal benthic organisms

Author(s)

Sellera, Fabio P. ; Fernandes, Miriam R. ; Moura, Quezia ; Lopes, Ralf B. ; Souza, Tiago A. ; Cerdeira, Louise ; Lincopan, Nilton

• Document type: Journal article
• Source: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 14, p. 2-pg., 2018-09-01.

Abstract

Objectives: Marine bivalves can act as bioindicators of marine environment pollution by multidrug-resistant (MDR) enteric bacteria of medical interest. The aim of this study was to report the draft genome sequence of a plasmid-encoded AmpC (pAmpC) (CMY-2)-carrying Escherichia coli isolate recovered from a marine bivalve sample in the coastal shore of Southeast Brazil. Methods: The whole genome was sequenced on an Illumina NextSeq platform and was assembled using Velvet v.1.2.10. Data analysis was carried out using tools available from the Center of Genomic Epidemiology and Geneious R10 software. Results: The genome size was calculated at 5198 055 bp, comprising a total of 5316 protein-coding sequences. The strain was assigned to ST457 and presented the bla(CMY-2) pAmpC gene. In addition, the strain was clustered into the pathogenic phylogenetic group D. Conclusion: The release of this draft genome sequence can provide valuable information to better understand the dissemination of MDR enteric bacteria in marine environments. (C) 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/13527-2 - Ancestral relationship, pan-resistoma plasmid of Escherichia coli producing b-lactamases of extended spectrum (CTX-M) and of plasmid curing methods
• Grantee: Miriam Rodriguez Fernandes
• Support Opportunities: Scholarships in Brazil - Doctorate