Photobiomodulation of local alterations induced by BthTX-I, a phospholipase A(2) myotoxin from Bothrops jararacussu snake venom: In vivo and in vitro evaluation

Author(s)

dos Santos, Adriano Silvio ; Guimaraes-Sousa, Ludmila ; Costa, Maricilia Silva ; Zamuner, Luis Fernando ; Sousa, Norma Cristina ; Hyslop, Stephen ; Soares, Andreimar M. ; Chavantes, Maria Cristina ; Cogo, Jose Carlos ; Zamuner, Stella Regina

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 107, p. 6-pg., 2018-02-01.

Abstract

This report describes the effect of photobiomodulation (PBM) on edema formation, leukocyte influx, prostaglandin E-2 (PGE(2)) biosynthesis and cytotoxicity caused by bothropstoxin-I (BthTX-I), a phospholipase A(2) (PLA(2)) homologue isolated from Bothrops jararacussu snake venom. Swiss mice or C2C12 cells were irradiated with low-level laser (LLL) at 685 nm wavelength, an energy density of 4.6 J/cm(2) and an irradiation time of 13 s. To evaluate the effect on edema formation and leukocyte influx, LLL was applied to the site of inoculation 30 min and 3 h post-injection. C2C12 cells were exposed to BthTX-I and immediately irradiated. PBM significantly reduced paw edema formation, peritoneal leukocyte influx and PGE(2) synthesis, but increased the viability of C2C12 muscle cells after BthTX-I incubation. These findings demonstrate that PBM attenuated the inflammatory events induced by BthTX-I. The attenuation of PGE(2) synthesis could be an important factor in the reduced inflammatory response caused by laser irradiation. The ability of LLL irradiation to protect muscle cells against the deleterious effects of BthTX-I may indicate preservation of the plasma membrane. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 07/07137-0 - Study of inflammatory reaction induced by Bothrops jararacussu snake venom and two miotoxins isolated by this venom
• Grantee: Ludmila Guimarães Souza
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/23757-0 - Effect of low level laser on C2C12 muscle cells subjected to injury by BthTX-I and II myotoxin islalated from Bothrops jararacussu snake venom
• Grantee: Adriano Silvio dos Santos
• Support Opportunities: Scholarships in Brazil - Master