Targeted-Analysis of beta-Carboline Alkaloids in Passionfruit ("Maracuja") by SBSE(PDMS)-LC/Flu and UHPLC-MS

Author(s)

Freire, Vitor F. ; Silva, Gabriela R. ; Yariwake, Janete H.

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 29, n. 4, p. 7-pg., 2018-04-01.

Abstract

Samples of pulp and seeds of sour (P. edulis) and sweet (P. alata) passionfruit were subjected to targeted analysis to identify beta-carboline alkaloids, using combined UHPLC-MS (ultrahigh performance liquid chromatography-mass spectrometry) and SBSE(PDMS) (stir bar sorption extraction using polydimethylsiloxane as stationary phase) for sample preconcentration. All the samples contained harmane and harmine; harmaline was found only in P. alata seeds and in P. edulis pulp, while harmalol was found only in P. alata seeds. An analysis of sweet passionfruit by SBSE(PDMS) combined with LC/Flu (liquid chromatography/fluorescence detection) indicated 1.03 x 10(-1) +/- 3.05 x 10(-3) mu g harmane L-1 pulp and 7.44 x 10(-5) +/- 2.55 x 10(-6) mu g harmane g(-1) seeds, but harmine was not detectable by the quantitative method. Our findings underscore the need for further studies about passionfruit food safety, in view of the potential toxicity of beta-carboline alkaloids. (AU)

• FAPESP's process: 09/54040-8 - Acquisition of a high-resolution Orbitrap mass spectrometer for the discovery and structural analysis of biologically active compounds: applications in proteomics, biomarkers, synthesis, isolation, and characterization of natural products, studies of redox systems in food and enzymatic synthesis
• Grantee: Emanuel Carrilho
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/21886-7 - Development of methods for identification and quantification of natural products in plant extracts, emphasizing SBSE techniques (SBSE/LC-Fluorescence and SBSE/UHPLC-MS) for the investigation of further passion fruit (maracujá) harmane alkaloids
• Grantee: Janete Harumi Yariwake
• Support Opportunities: Regular Research Grants