Genome-wide characterization of the NRAMP gene family in Phaseolus vulgaris provides insights into functional implications during common bean development

Author(s)

Ishida, Juliane Karine ; Caldas, Danielle G. G. ; Oliveira, Lucas Roberto ; Frederici, Gabriela Campos ; Pereira Leite, Lucas Margato ; Mui, Tsai Siu

• Document type: Journal article
• Source: GENETICS AND MOLECULAR BIOLOGY; v. 41, n. 4, p. 14-pg., 2018-10-01.

Abstract

Transporter proteins play an essential role in the uptake, trafficking and storage of metals in plant tissues. The Natural Resistance-Associated Macrophage Protein (NRAMP) family plays an essential role in divalent metal transport. We conducted bioinformatics approaches to identify seven NRAMP genes in the Phaseolus vulgaris genome, investigated their phylogenetic relation, and performed transmembrane domain and gene/protein structure analyses. We found that the NRAMP gene family forms two distinct groups. One group included the PvNRAMP1, -6, and -7 genes that share a fragmented structure with a numerous exon/intron organization and encode proteins with mitochondrial or plastidial localization. The other group is characterized by few exons that encode cytoplasmic proteins. In addition, our data indicated that PvNRAMP6 and -7 may be involved in mineral uptake and mobilization in nodule tissues, while the genes PvNRAMP1, -2, -3, -4 and -5 are potentially recruited during plant development. This data provided a more comprehensive understanding of the role of NRAMP transporters in metal homeostasis in P. vulgaris. (AU)

• FAPESP's process: 13/06301-2 - Deciphering the common bean resistance against the anthracnose disease: Transcriptome and identification of genes responsive to the compatible and incompatible interaction Phaseolus vulgaris/Colletotricuhm lindemuthianum
• Grantee: Danielle Gregorio Gomes Caldas
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/00359-7 - Visualization and quantification of Fusarium oxysporum f. sp. phaseoli in four varieties of bean plants (Phaseolus vulgaris L.)
• Grantee: Lucas Roberto de Oliveira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/00385-8 - Inhibitory effect of isolated bacteria from Amazonian dark earth on growth of Fusarium 14 oxysporum F. sp. Phaseoli
• Grantee: Gabriela Campos Frederici
• Support Opportunities: Scholarships in Brazil - Scientific Initiation