| Full text | |
| Author(s): |
Nora, Luisa Czamanski
;
Westmann, Caua Antunes
;
Martins-Santana, Leonardo
;
Alves, Luana de Fatima
;
Oliveira Monteiro, Lummy Maria
;
Guazzaroni, Maria-Eugenia
;
Silva-Rocha, Rafael
Total Authors: 7
|
| Document type: | Journal article |
| Source: | MICROBIAL BIOTECHNOLOGY; v. 12, n. 1, p. 23-pg., 2019-01-01. |
| Abstract | |
When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on both society and the scientific community. In the field of genetic engineering, the most important tool developed was the plasmid vector. This technology has been continuously expanding and undergoing adaptations. Here, we provide a detailed view following the evolution of vectors built throughout the years destined to study microorganisms and their peculiarities, including those whose genomes can only be revealed through metagenomics. We remark how synthetic biology became a turning point in designing these genetic tools to create meaningful innovations. We have placed special focus on the tools for engineering bacteria and fungi (both yeast and filamentous fungi) and those available to construct metagenomic libraries. Based on this overview, future goals would include the development of modular vectors bearing standardized parts and orthogonally designed circuits, a task not fully addressed thus far. Finally, we present some challenges that should be overcome to enable the next generation of vector design and ways to address it. (AU) | |
| FAPESP's process: | 16/05472-6 - Synthetic biology approaches to engineer/mine novel regulatory elements in bacteria |
| Grantee: | Cauã Antunes Westmann |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/06323-4 - New tools for improving metagenomic cellulase screenings |
| Grantee: | Luana de Fátima Alves |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/17924-1 - Construction of synthetic promoters for the expression of cellulases using novel cis-regulatory elements in Trichoderma reesei |
| Grantee: | Leonardo Martins Santana |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 16/19179-9 - Deciphering the architecture/function relationship in complex bacterial promoters through synthetic biology approaches |
| Grantee: | Lummy Maria Oliveira Monteiro |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/03763-3 - Unveiling the mechanisms of gene regulation at the single cell level in Trichoderma reesei |
| Grantee: | Luísa Czamanski Nora |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters |
| Grantee: | Rafael Silva Rocha |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries |
| Grantee: | María Eugenia Guazzaroni |
| Support Opportunities: | Research Grants - Young Investigators Grants |