| Grant number: | 16/05472-6 |
| Support type: | Scholarships in Brazil - Master |
| Effective date (Start): | September 01, 2016 |
| Effective date (End): | August 31, 2018 |
| Field of knowledge: | Biological Sciences - Microbiology - Biology and Physiology of Microorganisms |
| Principal researcher: | Rafael Silva Rocha |
| Grantee: | Cauã Antunes Westmann |
| Home Institution: | Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| Associated research grant: | 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters, AP.JP |
| Associated scholarship(s): | 17/16783-5 - Combining in silico and in vivo approaches for understanding the evolution of transcription factor binding sites in Escherichia coli, BE.EP.MS |
Abstract Despite the rapid expansion of Synthetic biology over the last decade, the field is being greatly limited by an informational bottleneck concerning biological data. Thus, it becomes essential to explore new molecular models from the perspective of the interrelationship between its components and the logic that emerges from these relationships in the system. In this context, this project proposes the study of the regulatory system of gene expression, a crucial mechanism for any organism that modulates its response to various stimuli. The main approach will be based on Escherichia coli studies under the panorama provided by Synthetic Biology. Synthetic promoter libraries will be assembled by random combination of operator sequences regarding two global transcription factors: FIS and IHF. The behaviour of the constructions will be analysed by fluorescence in single cells and populations, allowing a deeper insight into the architecture of complex promoters and their intrinsic logic. The expected results have great potential in elucidating the process of signals integration in complex promoters, allowing the transposition of the system`s logic into various biotechnological applications. | |