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Design of novel synthetic yeast promoters based on in silico approaches

Grant number: 18/06509-6
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2018
Effective date (End): December 31, 2019
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Rafael Silva Rocha
Grantee:Adriano Gomes Silva
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters, AP.JP

Abstract

Microorganisms of the yeast group are very important for several biotechnological applications. In recent years, several studies have focused on the engineering of synthetic circuits in yeasts aiming to both expand the metabolic and biosynthetic capacities of these organisms and increase their tolerance to aggressive environmental conditions such as those related to different industrial processes. In these works, it has been observed that a major limitation for the construction of synthetic circuits in yeast is the reduced number of modular and customizable regulatory elements that respond to the conditions of interest. Our group has been developing in recent years a series of computational and experimental approaches to the development of synthetic promoters in bacteria. In this project, we will cover the expansion of these approaches aiming at the in silico design and in vivo characterization of new synthetic promoters for Saccharomyces cerevisiae yeast. Thus, the main focus of the project will be the construction of new genetic algorithms that allow the combination of cis-regulatory elements for S. cerevisiae transcription factors. It is expected that new synthetic materials of biotechnological relevance will be obtained, as well as a new methodology for obtaining them. (AU)