Differential expression of RNA exosome subunits in the amphibian Lithobates catesbeianus during reproductive and non-reproductive periods

Author(s)

Luz, J. S. ; Caneguim, B. H. ; Baggio, A. ; Santoni, M. M. ; Helbing, C. C. ; Valentini, S. R. ; Sasso-Cerri, E. ; Oliveira, C. C.

• Document type: Journal article
• Source: BMC RESEARCH NOTES; v. 12, p. 7-pg., 2019-01-21.

Abstract

Objective: The RNA exosome is an evolutionarily conserved 3'-5' exoribonucleolytic protein complex involved in processing and degradation of different classes of nuclear and cytoplasmic RNAs, and, therefore, important for the posttranscriptional control of gene expression. Despite the extensive in vivo functional studies and the structural data on the RNA exosome, few studies have been performed on the localization and expression of exosome subunits during gametogenesis, process during which gene expression is largely controlled at the posttranscriptional level. Results: We report the identification of exosome subunits in Lithobates catesbeianus and analysis of the differential subcellular localization of RNA exosome core and catalytic subunits in testis cells. In addition, we show seasonal differences in the expression levels of four exosome subunits in different organs. In addition to being part of the RNA exosome complex, its subunits might participate independently of the complex in the control of gene expression during seasonal variation in bullfrog tissues. These results may be relevant for other eukaryotic species. (AU)

• FAPESP's process: 12/51200-7 - Identification and cellular localization of the exosome subunits in testis of Lithobates catesbeianus (Bullfrog)
• Grantee: Juliana Silva da Luz
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/23845-3 - Evaluation of Vitamin B12 effect on the mitotic and meiotic phases of spermatogenesis and in the sperm DNA of cimetidine-treated rats
• Grantee: Estela Sasso Cerri
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/21383-8 - Morphofunctional evaluation of bullfrog testes: a correlation with fat bodies and seasonality
• Grantee: Andressa Baggio Dias
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 10/51842-3 - Structural study of protein components of the exosome and some of its regulatory factors from Archaea and yeast
• Grantee: Carla Columbano de Oliveira
• Support Opportunities: Research Projects - Thematic Grants