Tailoring the Antimicrobial Response of Cationic Nanocellulose-Based Foams through Cryo-Templating

Author(s)

Otoni, Caio G. ; Figueiredo, Juliana S. L. ; Capeletti, Larissa B. ; Cardoso, Mateus B. ; Bernardes, Juliana S. ; Loh, Watson

• Document type: Journal article
• Source: ACS APPLIED BIO MATERIALS; v. 2, n. 5, p. 12-pg., 2019-05-20.

Abstract

To shed light on novel sustainable materials with antimicrobial functionality, in this contribution, we describe the use of cationic nanocellulose to produce foams featuring antibacterial activity against the powerful human pathogen Escherichia coli. Dialdehyde cellulose was cationized with Girard's reagent T (GRT), mechanically disintegrated into nanofibrillated cellulose (NFC), and shaped into foams through different protocols. All steps were carried out in aqueous media and in the absence of hazardous chemicals. While evaporative drying led to compact films (density of 1.3 g cm(-3)), freeze-casting (i.e., freezing and freeze-drying) produced monolithic cryogels with low densities (<50 mg cm(-3)) and porosities of ca. 98%. Although highly porous, the cryogels obtained through rapid freezing remarkably presented smaller pores than those that were previously frozen in a slow fashion. The quaternary ammonium groups of GRT-cationized NFC removed E. coli to different extents depending upon sample morphology. We demonstrated in an innovative manner that porosity, which is directly associated with surface area, and pore size play an essential role on the antimicrobial performance. This outcome arises from the inaccessibility of bacterial cells to cationic surfaces inside monoliths composed of small pores. We herein present an uncomplicated, environmentally friendly protocol for fine-tuning the porosity and pore size of all-cellulose materials through cryo-templating. Controlling these morphometric parameters allowed us to achieve a ca. 85% higher anti-E. coli activity when comparing samples made up of the very same material (i.e., the same NFC concentration and degree of substitution) but presented as dense films. These findings bear clear implications for the pursuit of sustainable materials presenting multifunctionality. (AU)

• FAPESP's process: 17/07013-1 - Surface modifications and polyelectrolyte adsorption via layer-by-layer onto cellulose nanofibers for the production of antimicrobial aerogels
• Grantee: Caio Gomide Otoni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/01167-7 - Silica nanoparticles functionalized with carbohydrate oligomers and their interaction with the bacterial membrane
• Grantee: Larissa Brentano Capeletti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral