Silica nanoparticles functionalized with carbohydrate oligomers and their interaction with the bacterial membrane

• Grant number: 17/01167-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2017
• End date: December 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Mateus Borba Cardoso
• Grantee: Larissa Brentano Capeletti
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated research grant: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles, AP.TEM

Abstract

Antibiotic resistant bacteria or superbugs are a real problem that has been already discussed and faced for some time. However, it remains actual as new resistance mechanisms emerge, making obsolete newly discovered drugs. The subject is of such importance that in the year 2016 it was discussed in the United Nations assembly, in an attempt of a worldwide commitment in the search of awareness, reduction and new strategies to combat these pathogens. Within this context, it is suggested the synthesis of silica nanoparticles (SiO2NPs), which may or may not have antibiotics encapsulated in the pores and will be functionalized with carbohydrates through the attachment of saccharide methacrylate oligomers such as sucrose, glucose and fructose. Bacteria are structures with low complexity and no specific receptors on their surface, so this functionalization aims an increasing of the SiO2NPs interaction with the bacteria membrane of susceptible and resistant strains through the outer layer composed of lipopolysaccharides. Consequently, an increase in efficacy against them may occur as a result of the nanoparticle targeting through carbohydrate-carbohydrate interactions. Therefore, SiO2NPs will be functionalized with methacrylate group, and subsequently polymerized with saccharide methacrylate monomers by different polymerization techniques such as conventional and controlled radical ATRP. Furthermore, the possibility of preventing non-specific adsorption of proteins present in blood plasma by carbohydrates will also be studied. The systems aiming the targeting of nanocarriers to the bacterial membrane will be developed in the present project and their chemical and structural properties, biological activity and antimicrobial effect will be studied. Thus, SiO2NPs coated with saccharides may represent a potential alternative in order to meet the need for new strategies in the fight against susceptible and antibiotic resistant bacteria. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OTONI, CAIO G.; FIGUEIREDO, JULIANA S. L.; CAPELETTI, LARISSA B.; CARDOSO, MATEUS B.; BERNARDES, JULIANA S.; LOH, WATSON. Tailoring the Antimicrobial Response of Cationic Nanocellulose-Based Foams through Cryo-Templating. ACS APPLIED BIO MATERIALS, v. 2, n. 5, p. 12-pg., 2019-05-20. (17/07013-1, 15/25406-5, 17/01167-7)

AFFONSO DE OLIVEIRA, JESSICA FERNANDA; CAPELETTI, LARISSA BRENTANO; CARDOSO, MATEUS BORBA. Are antibiotic-functionalized nanoparticles a promising tool in antimicrobial therapies?. Nanomedicine, v. 12, n. 23, p. 4-pg., 2017-12-01. (17/01167-7, 15/25406-5, 14/22322-2, 13/22429-9)

LOIOLA, LIVIA M. D.; BATISTA, MARINA; CAPELETTI, LARISSA B.; MONDO, GABRIELA B.; ROSA, RHUBIA S. M.; MARQUES, RAFAEL E.; BAJGELMAN, MARCIO C.; CARDOSO, MATEUS B.. Shielding and stealth effects of zwitterion moieties in double-functionalized silica nanoparticles. Journal of Colloid and Interface Science, v. 553, p. 540-548, OCT 1 2019. (17/09203-2, 15/25406-5, 16/21598-0, 17/01167-7, 17/06692-2, 16/16905-0)

LIVI, SEBASTIEN; LINS, LUANDA C.; CAPELETTI, LARISSA B.; CHARDIN, CHARLINE; HALAWANI, NOUR; BAUDOUX, JEROME; CARDOSO, MATEUS B.. Antibacterial surface based on new epoxy-amine networks from ionic liquid monomers. EUROPEAN POLYMER JOURNAL, v. 116, p. 56-64, JUL 2019. (17/11060-5, 17/26471-0, 15/25406-5, 17/01167-7)

CAPELETTI, LARISSA BRENTANO; AFFONSO DE OLIVEIRA, JESSICA FERNANDO; DIAS LOIOLA, LIVIA MESQUITA; GALDINO, FLAVIA ELISA; DA SILVA SANTOS, DENYS EWERTON; SOARES, THEREZA AMELIA; FREITAS, RAUL DE OLIVEIRA; CARDOSO, MATEUS BORBA. Gram-Negative Bacteria Targeting Mediated by Carbohydrate-Carbohydrate Interactions Induced by Surface-Modified Nanoparticles. ADVANCED FUNCTIONAL MATERIALS, v. 29, n. 48, NOV 2019. (17/01167-7, 13/22429-9, 15/25406-5, 18/09555-9, 16/21598-0)