Reduced Graphene Oxide Decorated with AuNPs as a New Aptamer-Based Biosensor for the Detection of Androgen Receptor from Prostate Cells

Author(s)

Crulhas, Bruno P. ; Basso, Caroline R. ; Parra, Joao P. R. L. L. ; Castro, Gustavo R. ; Pedrosa, Valber A.

• Document type: Journal article
• Source: JOURNAL OF SENSORS; v. 2019, p. 11-pg., 2019-01-01.

Abstract

Here we described an aptasensor based on graphene oxide (GO) decorated with gold nanoparticles (AuNPs) able to detect the AR released by prostate cells, in order to provide new insights about the relationship between AR and prostate cancer for a more precise diagnosis for cancer. Characterization of the surface modified GE was carried out by cyclic voltammetry and electrochemical impedance spectroscopy using ferrocene as a redox probe. Under the optimized experimental conditions, a detection limit of 0.5 ng/mL (3 sigma/slope) was obtained by the present electrochemical biosensor, along with a linear range of 0-110 ng/mL. By virtue of excellent sensitivity, specificity, and repeatability, the present electrochemical biosensor provides a potential application in clinical diagnosis. (AU)

• FAPESP's process: 16/25749-2 - Study of prostate cancer biomarkers by aptameric sensors
• Grantee: Bruno Pereira Crulhas
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/15919-8 - Monitoring of prostate cancer biomarkers using a biosensor
• Grantee: Valber de Albuquerque Pedrosa
• Support Opportunities: Regular Research Grants