Effects of Light Quality and Chemical Elicitors on the Growth Parameters and Rosmarinic Acid Content of in vitro Cultures of Hyptis pectinata (L.) Poit

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Pedroso, Rita C. N. ; Pimenta, Leticia. P. ; Tozatti, Marcos G. ; Branquinho, Nubia A. A. ; Hara, Alessandra C. B. A. M. ; Silva, Fernando H. L. ; Costa, Alan C. ; Silva, Fabiano G. ; Miranda, Carlos E. S. ; Silva, Marcio L. A. ; Cunha, Wilson R. ; Pauletti, Patricia M. ; Januario, Ana. H.

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 30, n. 11, p. 8-pg., 2019-11-01.

Abstract

Hyptis pectinata (L.) Poit is a folk medicinal species in Northeast Brazil. In this work, seedlings of H. pectinata were cultured in vitro under diverse light quality spectra and under the influence of two distinct chemical elicitors, salicylic acid and silver nitrate (SN), at concentrations of 30 and 60 mu M. Growth parameters, biomass and rosmarinic acid (RA) content were evaluated. RA may be associated with the functional properties of this plant. In the light quality experiments, the highest level of rosmarinic acid was found under red light (161.65 +/- 8.64 mu g g(-1) DW (dry weight)) at 20 days (4.26-fold higher than the control white light). Among the evaluated elicitors, elicitation with 60 mu M SN at 3 days and 30 mu M SN at 6 days favored the highest rosmarinic acid content (6361.70 +/- 216.96 and 4542.98 +/- 92.53 mu g g(-1) DW, respectively), with relatively high biomass yield and a similar number of leaves and height as those of control seedlings. This approach is new for H. pectinata. (AU)

• FAPESP's process: 11/00631-5 - Bioactive compounds from medicinal and food plants of the cerrado and Pantanal and their endophytic microbiota
• Grantee: Ana Helena Januário
• Support Opportunities: Regular Research Grants