Photoinactivation of Methicillin-Resistant S. Aureus Biofilm using a New Chlorin as Photosensitizer

Author(s)

Amaral, L. S. ; Linares, I. A. P. ; Perussi, J. R. ; Albella, P ; Raposo, M ; Andrews, D ; Ribeiro, P

• Document type: Journal article
• Source: PROCEEDINGS OF THE 8TH INTERNATIONAL CONFERENCE ON PHOTONICS, OPTICS AND LASER TECHNOLOGY (PHOTOPTICS); v. N/A, p. 5-pg., 2020-01-01.

Abstract

Due to the increase in bacterial resistance to antibiotics, the development of new drugs and technologies for the eradication of microorganisms is a priority. Photodynamic Therapy (PDT) depends on the interaction between a light-sensitive compound (photosensitizer), light, and molecular oxygen. The reaction generates reactive oxygen species (ROS), which induce cell death by oxidative stress. Antimicrobial Photodynamic Therapy (A-PDT) may be a promising alternative for microbial infections since its action occurs by multiple targets, which hinders the development of resistance. The main goal of this study was the evaluation of the potential of a newly synthesized chlorin derivative sterically prevented from self-aggregation as a photosensitizer to photoinactivation Methicillin-Resistant S. aureus (MRSA) biofilm and to investigate the membrane integrity after the treatment. The results showed a high potential of this chlorin for photoinactivation of MRSA biofilms reducing the survival index more than 5 log CFU mL(-1) leading to the unstructured membrane and consequent cell death by photooxidation of membrane components after A-PDT. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC