| Full text | |
| Author(s): |
Boldarine, Valter T.
;
Pedroso, Amanda P.
;
Brandao-Teles, Caroline
;
LoTurco, Edson G.
;
Nascimento, Claudia M. O.
;
Oyama, Lila M.
;
Bueno, Allain A.
;
Martins-de-Souza, Daniel
;
Ribeiro, Eliane B.
Total Authors: 9
|
| Document type: | Journal article |
| Source: | AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM; v. 319, n. 2, p. 11-pg., 2020-08-01. |
| Abstract | |
Menopause is often accompanied by visceral obesity. With the aim of exploring the consequences of ovarian failure on visceral fat, we evaluated the effects of ovariectomy and estrogen replacement on the proteome/phosphoproteome and on the fatty acid profile of the retroperitoneal adipose depot (RAT) of rats. Eighteen 3-mo-old female Wistar rats were either ovariectomized or sham operated and fed with standard chow for 3 mo. A subgroup of ovariectomized rats received estradiol replacement. RAT samples were analyzed with data-independent acquisitions LC-MS/MS, and pathway analysis was performed with the differentially expressed/ phosphorylated proteins. RAT lipid profile was analyzed by gas chromatography. Ovariectomy induced high adiposity and insulin resistance and promoted alterations in protein expression and phosphorylation. Pathway analysis showed that five pathways were significantly affected by ovariectomy, namely. metabolism of lipids (including fatty acid metabolism and mitochondrial fatty acid beta-oxidation), fatty acyl-CoA biosynthesis, innate immune system (including neutrophil degranulation), metabolism of vitamins and cofactors, and integration of energy metabolism (including ChREI3P activates metabolic gene expression). Lipid profile analysis showed increased palmitic and palmitoleic acid content. The analysis of the data indicated that ovariectomy favored lipogenesis whereas it impaired fatty acid oxidation and induced a proinflammatory state in the visceral adipose tissue. These effects are consistent with the findings of high adiposity, hyperleptinemia, and impaired insulin sensitivity. The observed alterations were partially attenuated by estradiol replacement. The data point to a role of disrupted lipid metabolism in adipose tissue in the genesis of obesity after menopause. (AU) | |
| FAPESP's process: | 12/03172-4 - Obesity and depression in the post-menopause: study in rats about the effects of ovariectomy on behavioral aspects and parameters of the central regulation of feeding |
| Grantee: | Eliane Beraldi Ribeiro |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/00098-7 - Multi-User Equipment approved in grant 2017/25588-1: cromatógrafo Acquity UPLC I-Class |
| Grantee: | Daniel Martins-de-Souza |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 14/10068-4 - Multi-User Equipment approved in grant 13/08711-3: mass spectrometer waters SYNAPT G2-Si HDMS + nanoACQUITY UPLC |
| Grantee: | Daniel Martins-de-Souza |
| Support Opportunities: | Multi-user Equipment Program |