Chemokines and cytokines profile in whole saliva of patients with periodontitis

Author(s)

Kawamoto, Dione ; Lucas Amado, Pamela Pontes ; Albuquerque-Souza, Emmanuel ; Bueno, Manuela Rocha ; Vale, Glauber Campos ; Saraiva, Luciana ; Alves Mayer, Marcia Pinto

• Document type: Journal article
• Source: CYTOKINE; v. 135, p. 9-pg., 2020-11-01.

Abstract

Clinical features suggest differences in immune response among periodontitis forms, albeit a large number of cytokines and chemokines remain to be evaluated. The saliva is an available source of mediators and its analysis would be valuable in order to understand pathophysiological differences. The objective of this study was analyze chemokines/cytokines profile in whole saliva of individuals with severe periodontitis (Stage III) presenting moderate [Grade B; GB] or rapid progression rate with a localized incisor-molar pattern [Grade C; GC/IMP]. A case-control study was designed for each periodontitis group. GB (n = 9) and GC/IMP (n = 7) patients and their healthy controls (C-GB, n = 9 and C-GC, n = 7) were evaluated. Non-stimulated saliva samples were assessed by a multiplex assay for a total of 40 cytokines, C-C and C-X-C motif chemokines. GC/IMP group presented higher levels of CCL17 and CCL27 (p = 0.04, FDR > 0.05), and lower levels of CCL2 (p = 0.04, FDR > 0.05) and CCL25 (p = 0.006, FDR < 0.05) when compared to its control. GB patients had higher levels of IL-6, IL-1 beta (p = 0.04, FDR > 0.05), and elevated pro-inflammatory (TNF-alpha,IL-1 beta,INF-gamma,IL-6, IL-16): anti-inflammatory (IL-2, IL-4, IL-10) ratio (p = 0.01, FDR < 0.05) compared to its control [p-values by Mann-Whitney test, and False Discovery Rate (FDR) by Benjamini-Hochburg corrections]. CCL-chemokines and cytokines contributed to differences between GC/C-GC and GB/C-GB, respectively (p < 0.05, PERMANOVA test). These preliminary data revealed that each periodontitis phenotype presented distinct immune profiles differentially expressed in saliva compared to their related controls, suggesting differences in the etiopathogenesis of GB and GC/IMP. (AU)

• FAPESP's process: 16/13159-6 - Supra and subgingival microbiota diversity analysis and protein profile of saliva and enamel film of individuals with chronic periodontitis and microbial succession
• Grantee: Dione Kawamoto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/16377-7 - EVALUATION OF THE EFFECT OF PROBIOTIC BACTERIA ON THE IMUNE RESPONSE TO A.ACTINOMYCETEMCOMITANS: A STUDY IN GINGIVAL EPITHELIAL CELLS AND IN EXPERIMENTAL MODEL OF INFECTION IN VIVO.
• Grantee: Manuela Rocha Bueno
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/00259-0 - Microbial diversity of supra and subgingival biofilms and protein profile of saliva and acquired pellicle of subjects with aggressive periodontitis
• Grantee: Pâmela Pontes Penas Amado
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/10829-3 - Immunomodulatory effect of probiotic bacteria: analysis in peripheral monocytes and dendritic cells.
• Grantee: Gláuber Campos Vale
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/18273-9 - New strategies for the control of periodontitis
• Grantee: Marcia Pinto Alves Mayer
• Support Opportunities: Research Projects - Thematic Grants