Lignans as new chemical markers of a certified Brazilian organic propolis

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Author(s):	Tiveron, Ana Paula ; Rosalen, Pedro Luiz ; Ferreira, Antonio Gilberto ; Thomasi, Sergio Scherrer ; Massarioli, Adna Prado ; Ikegaki, Masaharu ; Franchin, Marcelo ; de Oliveira Sartori, Alan Giovanini ; de Alencar, Severino Matias Total Authors: 9
Document type:	Journal article
Source:	NATURAL PRODUCT RESEARCH; v. 36, n. 8, p. 5-pg., 2020-10-24.
Abstract
Commercially certified organic propolis produced in areas of environmental conservation and reforestation forests of Southern Brazil are generally poor in flavonoids, although one of its variants - Brazilian certified organic propolis 1 (OP1) - has shown strong antioxidant activity. The objective was to identify active compounds from OP1 related to its strong antioxidant activity. OP1 ethanolic extracts were subjected to liquid-liquid fractionation, and the fractions presenting the strongest antioxidant activity were combined and purified into subfractions. Compounds isolated from the most active subfractions had their structure elucidated by Nuclear Magnetic Resonance (NMR). As a result, five lignans and two lignan-precursors were isolated, and four of them are herein reported for the very first time in propolis. Hence, these compounds may be used as chemical markers for product standardization and authentication purposes, since OP1 is only produced by honeybees in native forests and its botanical origins remain unknown. (AU)