Cytotoxicity and Antimicrobial Properties of Photosynthesized Silver Chloride Nanoparticles Using Plant Extract from Stryphnodendron adstringens (Martius) Coville

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Fernandes, Diogenes G. da S. ; Andrade, Viviane Brito ; Lucena, Leticia Neves ; Ambrosio, Felipe Nogueira ; de Souza, Andre L. M. ; Batista, Bruno L. ; Rolim, Wallace Rosado ; Seabra, Amedea Barozzi ; Lombello, Christiane B. ; da Silva, Fernanda Dias ; Garcia, Wanius

• Document type: Journal article
• Source: JOURNAL OF CLUSTER SCIENCE; v. 33, n. 2, p. 9-pg., 2021-02-05.

Abstract

Stryphnodendron adstringens (Martius) Coville is a medicinal plant described as having pharmacological properties as anti-inflammatory and antimicrobial activities. Silver chloride nanoparticles (AgCl-NPs) have shown great potential for biomedical applications with efficient antimicrobial properties. Here, we report the photosynthesis of AgCl-NPs using plant extract from S. adstringens (SaAgCl-NPs) and their cytotoxic and antimicrobial activities. We photosynthesized SaAgCl-NPs nearly spherical with low heterogeneity in size and enveloped by an organic material layer responsible for colloidal stability. SaAgCl-NPs was non-cytotoxic against mammalian VERO cells; however, SaAgCl-NPs presented remarkable antifungal activity against the pathogenic yeast Cryptococcus neoformans (MIC80 of 0.32 mu g/mL) the causative agent of human cryptococcosis. Notable antibacterial activity was observed against Gram-negative bacteria Pseudomonas aeruginosa (MIC80 of 2.56 mu g/mL) e Serratia marcescens (MIC80 of 20.48 mu g/mL) both microorganisms associated with a variety of human infections, in particular pneumonia. In contrast, Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, microorganisms that cause pathologies as skin infections, were less susceptible to the SaAgCl-NPs both with MIC80 of 40.93 mu g/mL. Thus, SaAgCl-NPs represents an organic-inorganic hybrid nanomaterial with very low cytotoxicity against mammalian cells and high antimicrobial efficiency against pathogenic microorganisms and may be explored as an alternative to antimicrobial drugs. (AU)

• FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/17275-3 - Studies of the mode of action of two lytic polysaccharide monooxygenases from insect (order: isoptera): molecular structure, bioinorganic chemistry and biotechnological applications
• Grantee: Wanius José Garcia da Silva
• Support Opportunities: Regular Research Grants