NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress

Author(s)

Gavassi, Marina Alves ; Silva, Giselle Schwab ; Santiago da Silva, Carolina de Marchi ; Thompson, Andrew J. ; Macleod, Kyle ; Rayner Oliveira, Paulo Marcelo ; Cavalheiro, Mariana Feitosa ; Domingues, Douglas Silva ; Habermann, Gustavo

• Document type: Journal article
• Source: ENVIRONMENTAL AND EXPERIMENTAL BOTANY; v. 185, p. 12-pg., 2021-02-06.

Abstract

Aluminum (Al)-induced decrease in leaf hydration has been associated with low gas exchange, especially stomatal conductance (g(s)). However, the mechanisms explaining these responses are unclear. Citrus limonia was exposed to 0 and 1480 mu M Al in nutrient solution for 90 days to test whether the low g(s) and leaf hydration in plants exposed to Al is associated with increased 9-cis-epoxycarotenoid dioxygenase (NCED) gene expression and abscisic acid (ABA) biosynthesis. Relative leaf water content (RWC), water potential (Psi w) and gas exchange in the leaves, as well as leaf and root ClNCED3, ClNCED1 and ClNCED5 expression and accumulation of ABA and its metabolites (phaseic acid, dihydrophaseic acid, (+)-7'-hydroxy-ABA and ABA-beta-D-glucosyl ester) were measured. Aluminum up-regulated ClNCED3 and induced ABA accumulation in the roots before impairments in leaf water status (low Psi w, RWC and g(s)) could be observed. Leaf ABA concentration increased from 7 to 90 days and this could be partially explained by the up-regulation of ClNCED3, ClNCED1 and ClNCED5 in this organ. Stomatal closure occurred concomitantly with the increase of ABA concentration, and this result provides further evidence of the role of ABA modulation of plant hydration under Al stress. (AU)

• FAPESP's process: 16/10896-0 - Can hexanoic acid signaling modulate the transcriptome, metabolome and pathogen development in coffee trees?
• Grantee: Douglas Silva Domingues
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/08902-7 - ABA signaling for stomatal closure under Al stress
• Grantee: Marina Alves Gavassi
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/26144-0 - Hydraulic, metabolic and molecular aspects of Al3+ interference in above ground plant hydration in Citrus limonia
• Grantee: Gustavo Habermann
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/25658-2 - Aluminum interference in leaf hydration in citrus limonia and in photosynthetic performance of Vochysia tucanorum
• Grantee: Giselle Schwab Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/25409-4 - Role of ABA in water relations in metabolisms with contrasting sensitivities to Al3+
• Grantee: Marina Alves Gavassi
• Support Opportunities: Scholarships in Brazil - Doctorate