Effect of phosphorylated chitosan and carbodiimide biomodification on the chemical composition of eroded dentin

Author(s)

Lopes Ururahy, Mirian Saavedra ; Curylofo-Zotti, Fabiana Almeida ; Costa Lizarelli, Gustavo Teodoro ; dos Reis, Arlete Barbosa ; Ramos, Ana Paula ; Milori Corona, Silmara Aparecida

• Document type: Journal article
• Source: AMERICAN JOURNAL OF DENTISTRY; v. 34, n. 2, p. 5-pg., 2021-04-01.

Abstract

Purpose: To evaluate the chemical composition and morphological properties of eroded dentin after biomodification with phosphorylated chitosan (P-Chi) and carbodiimide (EDC). Methods: 42 bovine dentin specimens were used; 21 of these specimens were subjected to erosive challenge with 0.3% citric acid (pH = 3.2) for 2 hours. The specimens were randomly divided into six groups according to dentin substrate (sound or eroded) and biomodification [with 2.5% P-Chi, with 0.5 mol/L EDC, or no biomodification (control)]. The specimens were analyzed by Fourier-transform infrared spectroscopy (FTIR, n= 5, in triplicate) and atomic force microscopy (AFM, n= 2) to verify the phosphate, carbonate, and organic matrix absorption peaks and to investigate surface morphology, respectively. The data were analyzed with Origin 6.0. Results: Dentin erosion reduced the intensity of the phosphate (1,100 cm-1) and carbonate (872 cm-1) related bands, which evidenced demineralization. Eroded dentin consisted of a more irregular surface containing slightly more open tubules. Modification with P-Chi removed intertubular dentin, which was compatible with surface demineralization; however, this modification obliterated dentin tubules. EDC did not promote demineralization. Biomodified dentin had a more irregular surface, irrespective of substrate type. (AU)

• FAPESP's process: 17/27134-8 - Effect of biomodified solutions phosphorylated chitosan and carbodiimide on chemical composition of eroded dentin
• Grantee: Gustavo Teodoro Costa Lizarelli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation