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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of Extraction Parameters for the Analysis of Lipid Classes in Plants

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Author(s):
Taynara S. Matos [1] ; Mariana S. Marques [2] ; Cleber J. N. Chaves [3] ; Flávia S. Zandonadi [4] ; Clarisse Palma-Silva [5] ; Alessandra Sussulini
Total Authors: 6
Affiliation:
[1] Universidade Estadual de Campinas (UNICAMP). Instituto de Química. Departamento de Química Analítica - Brasil
[2] Universidade Estadual de Campinas (UNICAMP). Instituto de Química. Departamento de Química Analítica - Brasil
[3] Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia. Laboratório de Ecologia Molecular e Genômica Evolutiva de Plantas - Brasil
[4] Universidade Estadual de Campinas (UNICAMP). Instituto de Química. Departamento de Química Analítica - Brasil
[5] Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia. Laboratório de Ecologia Molecular e Genômica Evolutiva de Plantas - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 34, n. 11, p. 1631-1640, 2023-10-23.
Abstract

The aim of this study was to preliminary evaluate the lipid profile alterations on Pitcairnia flammea leaves based on variations in solvent proportion and ultrasonic ice bath extraction time, followed by a lipid class-enriched analysis employing chemometric techniques. Ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw data and MS-DIAL and MetaboAnalyst platforms were used to perform data preprocessing and statistical analysis. The statistical analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization of a trend distribution based on extraction time. Furthermore, we were able to establish that the solvent proportion had a greater impact on group separation in data samples extracted for 30 min versus 10 and 20 min. Moreover, diacylglycerol or/and lysophosphatidylcholine are lipid subclasses that can be favored depending on the extraction time in the mass spectrometry analyses using positive electrospray ionization mode. (AU)

FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Marco Aurelio Zezzi Arruda
Support Opportunities: Research Projects - Thematic Grants