Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

Author(s):Show less -

Tatiana MARTINI ^[1] ; Daniela RIOS ^[2] ; Aline DIONIZIO ^[3] ; Luiza de Paula Silva CASSIANO ^[4] ; Cíntia Maria de Souza e SILVA ^[5] ; Even Akemi TAIRA ^[6] ; Talita Mendes Oliveira VENTURA ^[7] ; João Victor Frazão CÂMARA ^[8] ; Tamara Teodoro Araujo ^[9] ; Ana Carolina MAGALHÃES ^[10] ; Thiago Saads CARVALHO ^[11] ; Tommy BAUMANN ^[12] ; Adrian LUSSI ^[13] ; Ricardo Brandt de OLIVEIRA ^[14] ; Regina Guenka PALMA-DIBB ^[15] ; Marília Afonso Rabelo BUZALAF ^[16]

Affiliation:Show less -

^[1] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[2] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[3] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[4] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[5] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[6] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[7] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[8] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[9] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[10] Universidade de São Paulo. Bauru School of Dentistry - Brasil

^[11] University of Bern. Department of Preventive, Restorative and Pediatric Dentistry - Suíça

^[12] University of Bern. Department of Preventive, Restorative and Pediatric Dentistry - Suíça

^[13] University of Bern. Department of Preventive, Restorative and Pediatric Dentistry - Suíça

^[14] Universidade de São Paulo. Ribeirão Preto School of Medicine. Department of General Medicine - Brasil

^[15] Universidade de São Paulo. Ribeirão Preto School of Medicine. Department of Restorative Dentistry - Brasil

^[16] Universidade de São Paulo. Bauru School of Dentistry - Brasil

• Document type: Journal article
• Source: Brazilian Oral Research; v. 37, 2023-09-15.

Abstract

Abstract The objective of this study was to compare the protein profile of the acquired enamel pellicle (AEP) formed in vivo in patients with or without gastroesophageal reflux disease (GERD), and with or without erosive tooth wear (ETW). Twenty-four volunteers were divided into 3 groups: 1) GERD and ETW; 2) GERD without ETW; and 3) control (without GERD). The AEP formed 120 min after prophylaxis was collected from the lingual/palatal surfaces. The samples were subjected to mass spectrometry (nLC-ESI-MS/MS) and label-free quantification by Protein Lynx Global Service software. A total of 213 proteins were identified, or 119, 92 and 106 from each group, respectively. Group 2 showed a high number of phosphorylated and calcium-binding proteins. Twenty-three proteins were found in all the groups, including 14-3-3 protein zeta/delta and 1-phosphatidylinositol. Several intracellular proteins that join saliva after the exfoliation of oral mucosa cells might have the potential to bind hydroxyapatite, or participate in forming supramolecular aggregates that bind to precursor proteins in the AEP. Proteins might play a central role in protecting the dental surface against acid dissolution. (AU)

• FAPESP's process: 18/12041-7 - Safety and efficacy for the prevention of dental erosion of a new cystatin derived from sugarcane (CaneCPI-5): in vitro, in situ and in vivo studies
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/17977-8 - PROTEOMICS OF ACQUIRED ENAMEL PELLICLE AND SALIVA IN VOLUNTEERS WITH GASTROESOPHAGEAL REFLUX WITH DENTAL EROSION OR NOT AND IN VITRO EVALUATION OF PROTECTIVE POTENTIAL OF HEMOGLOBIN AGAINST DENTAL EROSION
• Grantee: Tatiana Martini
• Support Opportunities: Scholarships in Brazil - Doctorate