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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples

Full text
Author(s):
Joice Lara Maia Faria ; Ana Sílvia Dagnone [2] ; Thiago Demarchi Munhoz [3] ; Carolina Franchi João [4] ; Wanderson Adriano Biscola Pereira [5] ; Rosângela Zacarias Machado [6] ; Mirela Tinucci-Costa [7]
Total Authors: 7
Document type: Journal article
Source: Revista Brasileira de Parasitologia Veterinária; v. 19, n. 2, p. 98-102, 2010-06-00.
Abstract

The aim of this study was to compare the detection of Ehrlichia canis morulae and DNA by nPCR in whole blood and spleen aspiration. The sample included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis. Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears. E. canis DNA was detected in 29/40 blood samples. We verified that morulae detection is more efficient in cytological preparations from spleen aspiration. On the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosis. (AU)