Leishmaniinae: Evolutionary inferences based on protein expression profiles (PhyloQuant) congruent with phylogenetic relationships among Leishmania, Endotrypanum, Porcisia, Zelonia, Crithidia, and Leptomonas

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Mule, Simon Ngao ; Aleman, Evaristo Villalba ; Rosa-Fernandes, Livia ; Saad, Joyce S. ; de Oliveira, Gilberto Santos ; Martins, Deivid ; Angeli, Claudia Blanes ; Brandt-Almeida, Deborah ; Cortez, Mauro ; Larsen, Martin Rossel ; Shaw, Jeffrey J. ; Teixeira, Marta M. G. ; Palmisano, Giuseppe

• Document type: Journal article
• Source: PROTEOMICS; v. 24, n. 18, p. 17-pg., 2024-06-08.

Abstract

Evolutionary relationships among parasites of the subfamily Leishmaniinae, which comprises pathogen agents of leishmaniasis, were inferred based on differential protein expression profiles from mass spectrometry-based quantitative data using the PhyloQuant method. Evolutionary distances following identification and quantification of protein and peptide abundances using Proteome Discoverer and MaxQuant software were estimated for 11 species from six Leishmaniinae genera. Results clustered all dixenous species of the genus Leishmania, subgenera L. (Leishmania), L. (Viannia), and L. (Mundinia), sister to the dixenous species of genera Endotrypanum and Porcisia. Placed basal to the assemblage formed by all these parasites were the species of genera Zelonia, Crithidia, and Leptomonas, so far described as monoxenous of insects although eventually reported from humans. Inferences based on protein expression profiles were congruent with currently established phylogeny using DNA sequences. Our results reinforce PhyloQuant as a valuable approach to infer evolutionary relationships within Leishmaniinae, which is comprised of very tightly related trypanosomatids that are just beginning to be phylogenetically unraveled. In addition to evolutionary history, mapping of species-specific protein expression is paramount to understand differences in infection processes, tissue tropisms, potential to jump from insects to vertebrates including humans, and targets for species-specific diagnostic and drug development. (AU)

• FAPESP's process: 21/14751-4 - Novel mass spectrometry-based technologies for post-translational modifications analysis
• Grantee: Simon Ngao Mule
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/23689-2 - Proteomic and post-translation modification characterization of Leishmanias L. (V.) braziliensis, L. (L.) amazonensis and L. (L.) chagasi using a shotgun protemics approaches.
• Grantee: Joyce Silva Saad
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 20/04923-0 - SARS-CoV-2 glycosylation: a blueprint structural insight for understanding COVID-19 pathogenesis
• Grantee: Giuseppe Palmisano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins
• Grantee: Giuseppe Palmisano
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications
• Grantee: Giuseppe Palmisano
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 17/04032-5 - Dissecting the pathogenesis of Chagas Disease by deep glycomics and glycoproteomics approaches
• Grantee: Simon Ngao Mule
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/13562-0 - Unveiling the role of the immune checkpoint ligand CD200 in the host infection by Leishmania spp.
• Grantee: Mauro Javier Cortez Véliz
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/14179-9 - The role of the endoplasmic reticulum in viral and parasitic virulence
• Grantee: Deivid Martins Santos
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/13283-4 - Discovery of biomarkers of Chagas' disease in urine using mass spectrometry techniques
• Grantee: Gilberto Santos de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate