SARS-CoV-2 glycosylation: a blueprint structural insight for understanding COVID-19 pathogenesis

• Grant number: 20/04923-0
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2020
• End date: April 30, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Giuseppe Palmisano
• Grantee: Giuseppe Palmisano
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Carla Claser ; Carsten Wrenger ; Cláudio Romero Farias Marinho ; Danielle Bruna Leal de Oliveira ; Edison Luiz Durigon ; Edmarcia Elisa de Souza ; Fábio de Rose Ghilardi ; Lívia Rosa Fernandes ; Rinaldo Focaccia Siciliano
• Associated research grant: 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications, AP.JP2
• Associated scholarship(s): 21/00507-4 - Glycosylation of SARS-CoV-2 to identify the structural characteristics of COVID-19, BP.PD

Abstract

Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) is an emerging human virus from the Coronaviridae family. SARS-CoV-2 is the etiological agent of novel pneumonia called COVID-19, coronavirus disease 2019. Due to the rapid increase in the number of cases that have affected countries, WHO declared COVID-19 outbreak an ongoing pandemic. As of April 1st, there are 750,890 confirmed cases and 36,405 deaths globally. Understanding host-virus dynamics is critical for development of prophylactic, diagnostic and therapeutic strategies and epidemiological control of COVID-19. Many features are yet to be understood on SARS-CoV-2 behavior and host response during infection, e.g. the molecular and cellular traits of severe, mild and asymptomatic infections, providing higher understanding of the pathogenesis. In this project we propose the application of proteome-wide spatial and temporal approach of SARS-CoV-2:host interaction, to be addressed in three interconnected fronts: (1) Assessment of activated signaling pathways by systematic quantitative analysis of temporal dynamics in protein and glycosylation changes during SARS-CoV-2 infection of human cells; (2) characterization of proteome and PTMs diversity of SARS-CoV-2; and (3) development of an early diagnostic and prognostic platform based on the proteomic profile of sera from patients with different clinical characteristics. The project proposed will contribute to understand the viral pathogenesis and prioritize biomarkers for rapid diagnostic tests. (AU)

Scientific publications

(19)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MULE, SIMON NGAO; SAAD, JOYCE SILVA; FERNANDES, LIVIA ROSA; STOLF, BEATRIZ S.; CORTEZ, MAURO; PALMISANO, GIUSEPPE. Protein glycosylation inLeishmaniaspp.. MOLECULAR OMICS, v. 16, n. 5, p. 407-424, OCT 1 2020. (20/04923-0, 18/15549-1, 16/23689-2, 17/04032-5)

SUMAM DE OLIVEIRA, DAFFINY; KRONENBERGER, THALES; PALMISANO, GIUSEPPE; WRENGER, CARSTEN; DE SOUZA, EDMARCIA ELISA. Targeting SUMOylation in Plasmodium as a Potential Target for Malaria Therapy. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JUN 10 2021. (18/10150-3, 20/04923-0, 19/26771-0, 20/12277-0, 17/03966-4, 18/18257-1, 18/15549-1, 15/26722-8)

DOMBROWSKI, JAMILLE GREGORIO; BARATEIRO, ANDRE; MACHADO PEIXOTO, ERIKA PAULA; CLAUDIO DA SILVA BARROS, ANDRE BOLER; DE SOUZA, RODRIGO MEDEIROS; CLARK, TAANE GREGORY; CAMPINO, SUSANA; WRENGER, CARSTEN; WUNDERLICH, GERHARD; PALMISANO, GIUSEPPE; et al. Adverse pregnancy outcomes are associated with Plasmodium vivax malaria in a prospective cohort of women from the Brazilian Amazon. PLoS Neglected Tropical Diseases, v. 15, n. 4, APR 2021. (17/03966-4, 17/03939-7, 18/18257-1, 19/12068-5, 15/26722-8, 17/24267-7, 20/06747-4, 18/20468-0, 20/04923-0, 18/15549-1, 17/05782-8)

MULE, SIMON NGAO; GOMES, VINICIUS DE MORAIS; WAILEMANN, ROSANGELA A. M.; MACEDO-DA-SILVA, JANAINA; ROSA-FERNANDES, LIVIA; LARSEN, MARTIN R.; LABRIOLA, LETICIA; PALMISANO, GIUSEPPE. HSPB1 influences mitochondrial respiration in ER-stressed beta cells. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1869, n. 9, SEP 2021. (17/04032-5, 14/02745-6, 17/03618-6, 18/18257-1, 16/04676-7, 20/04923-0, 16/14845-0, 13/07937-8, 18/15549-1, 21/00507-4)

CARNEIRO, ANDREIA; VIANA-GOMES, DIEGO; MACEDO-DA-SILVA, JANAINA; OLIVEIRA LIMA, GISCARD HUMBERTO; MITRI, SIMONE; ALVES, SERGIO RABELLO; KOLLIARI-TURNER, ALEXANDER; ZANOTELI, EDMAR; DE AQUINO NETO, FRANCISCO RADLER; PALMISANO, GIUSEPPE; et al. Risk factors and future directions for preventing and diagnosing exertional rhabdomyolysis. Neuromuscular Disorders, v. 31, n. 7, p. 583-595, JUL 2021. (20/04923-0, 18/15549-1, 18/18257-1)

FLOREZ-ALVAREZ, LIZDANY; DE SOUZA, EDMARCIA ELISA; BOTOSSO, VIVIANE FONGARO; LEAL DE OLIVEIRA, DANIELLE BRUNA; HO, PAULO LEE; TABORDA, CARLOS PELLESCHI; PALMISANO, GIUSEPPE; CAPURRO, MARGARETH LARA; REBELLO PINHO, JOAO RENATO; FERREIRA, HELENA LAGE; et al. Hemorrhagic fever viruses: Pathogenesis, therapeutics, and emerging and re-emerging potential. FRONTIERS IN MICROBIOLOGY, v. 13, p. 16-pg., 2022-10-25. (16/20045-7, 19/12303-4, 18/18257-1, 20/04923-0, 17/03966-4, 17/27131-9, 22/01812-8, 18/15549-1, 15/26722-8, 20/12277-0, 20/06409-1, 16/08730-6)

CARNEIRO, ANDREIA; MACEDO-DA-SILVA, JANAINA; SANTIAGO, VERONICA FEIJOLI; DE OLIVEIRA, GILBERTO SANTOS; GUIMARAES, THIAGO; MENDONCA, CLARISSA FEROLLA; DE OLIVEIRA BRANQUINHO, JESSICA LAIS; LUCENA, CINTIA VERDAN; OSORIO, JULIANA; PERNAMBUCO, EDUARDO; et al. Urine proteomics as a non-invasive approach to monitor exertional rhabdomyolysis during military training. JOURNAL OF PROTEOMICS, v. 258, p. 17-pg., 2022-04-30. (18/13283-4, 14/27198-8, 18/18257-1, 20/04923-0, 18/15549-1, 21/00140-3)

LAZARI, LUCAS CARDOSO; GHILARDI, FABIO DE ROSE; ROSA-FERNANDES, LIVIA; ASSIS, DIEGO M.; NICOLAU, JOSE CARLOS; SANTIAGO, VERONICA FEIJOLI; DALCOQUIO, TALIA FALCAO; ANGELI, CLAUDIA B.; BERTOLIN, ADRIADNE JUSTI; MARINHO, CLAUDIO R. F.; et al. Prognostic accuracy of MALDI-TOF mass spectrometric analysis of plasma in COVID-19. LIFE SCIENCE ALLIANCE, v. 4, n. 8, AUG 2021. (20/04705-2, 18/15549-1, 17/03966-4, 15/26722-8, 18/20468-0, 20/04923-0, 18/18257-1)

MULE, SIMON NGAO; ROSA-FERNANDES, LIVIA; COUTINHO, JOAO V. P.; GOMES, VINICIUS DE MORAIS; MACEDO-DA-SILVA, JANAINA; SANTIAGO, VERONICA FEIJOLI; QUINA, DANIEL; DE OLIVEIRA, GILBERTO SANTOS; THAYSEN-ANDERSEN, MORTEN; LARSEN, MARTIN R.; et al. Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay. JOURNAL OF PROTEOMICS, v. 248, SEP 30 2021. (18/15549-1, 13/07937-8, 20/04923-0, 17/04032-5, 18/18257-1)

RIBEIRO, GIOVANE DE JESUS GOMES; YAN, SUN LIU REI; PALMISANO, GIUSEPPE; WRENGER, CARSTEN. Plant Extracts as a Source of Natural Products with Potential Antimalarial Effects: An Update from 2018 to 2022. PHARMACEUTICS, v. 15, n. 6, p. 19-pg., 2023-06-01. (18/15549-1, 18/18257-1, 20/04923-0, 17/26358-0, 15/26722-8)

LAZARI, LUCAS C.; ZERBINATI, RODRIGO M.; ROSA-FERNANDES, LIVIA; SANTIAGO, VERONICA FEIJOLI; ROSA, KLAISE F.; ANGELI, CLAUDIA B.; SCHWAB, GABRIELA; PALMIERI, MICHELLE; SARMENTO, DMITY J. S.; MARINHO, CLAUDIO R. F.; et al. ALDI-TOF mass spectrometry of saliva samples as a prognostic tool for COVID-1. OURNAL OF ORAL MICROBIOLOG, v. 14, n. 1, DEC 31 2022. (18/15549-1, 20/06409-1, 20/04923-0, 18/18257-1, 15/26722-8, 17/03966-4, 21/07490-0, 18/20468-0)

SOUZA JUNIOR, DOUGLAS RICARDO; MARTINS SILVA, AMANDA RIBEIRO; ROSA-FERNANDES, LIVIA; REIS, LORENNA ROCHA; ALEXANDRIA, GABRIELLY; BHOSALE, SANTOSH D.; GHILARDI, FABIO DE ROSE; DALCOQUIO, TALIA FALCAO; BERTOLIN, ADRIADNE JUSTI; NICOLAU, JOSE CARLOS; et al. HDL proteome remodeling associates with COVID-19 severity. JOURNAL OF CLINICAL LIPIDOLOGY, v. 15, n. 6, p. 796-804, NOV-DEC 2021. (15/26722-8, 16/00696-3, 20/04923-0, 19/25702-4, 17/07725-1, 18/15549-1, 18/18257-1, 13/07937-8)

ROSA-FERNANDES, LIVIA; BANDEIRA, CARLA; POUR, SHAHAB ZAKI; BENEDETTI, VIVIANE DE FATIMA; FERREIRA, DANIEL; LORENZON, ALINE R.; MORELI, JUSCIELE B.; MARINHO, CLAUDIO ROMERO FARIAS; LARSEN, MARTIN R.; ZANOTTO, PAOLO; et al. The impact of Zika virus exposure on the placental proteomic profile. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, v. 1868, n. 1, JAN 1 2022. (20/04923-0, 18/18257-1)

SAN FRANCISCO, JUAN; ASTUDILLO, CONSTANZA; LUIS VEGA, JOSE; CATALAN, ALEJANDRO; GUTIERREZ, BESSY; ARAYA, JORGE E.; ZAILBERGER, ANIBAL; MARINA, ANABEL; GARCIA, CARLOS; SANCHEZ, NURIA; et al. Trypanosoma cruzi pathogenicity involves virulence factor expression and upregulation of bioenergetic and biosynthetic pathways. VIRULENCE, v. 13, n. 1, p. 22-pg., 2022-12-31. (18/15549-1, 20/04923-0, 18/18257-1, 21/00140-3)

MACEDO-DA-SILVA, JANAINA; SANTIAGO, VERONICA F.; ROSA-FERNANDES, LIVIA; MARINHO, CLAUDIO R. F.; PALMISANO, GIUSEPPE. Protein glycosylation in extracellular vesicles: Structural characterization and biological functions. Molecular Immunology, v. 135, p. 226-246, JUL 2021. (18/15549-1, 18/20468-0, 18/18257-1, 20/04923-0)

CARRASCO, ALEXIS GERMAN MURILLO; OTAKE, ANDREIA HANADA; MACEDO-DA-SILVA, JANAINA; SANTIAGO, VERONICA FEIJOLI; PALMISANO, GIUSEPPE; ANDRADE, LUCIANA NOGUEIRA DE SOUSA; CHAMMAS, ROGER. Deciphering the Functional Status of Breast Cancers through the Analysis of Their Extracellular Vesicles. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 16, p. 33-pg., 2023-08-01. (18/15549-1, 18/18257-1, 20/04923-0, 21/00140-3, 19/05583-0)

MORETTI, ISABELE FATTORI; LERARIO, ANTONIO MARCONDES; SOLA, PAULA RODRIGUES; MACEDO-DA-SILVA, JANAINA; BAPTISTA, MAURICIO DA SILVA; PALMISANO, GIUSEPPE; OBA-SHINJO, SUELI MIEKO; MARIE, SUELY KAZUE NAGAHASHI. GBM Cells Exhibit Susceptibility to Metformin Treatment According to TLR4 Pathway Activation and Metabolic and Antioxidant Status. CANCERS, v. 15, n. 3, p. 18-pg., 2023-02-01. (20/04923-0, 18/18257-1, 04/12133-6, 20/02988-7, 21/00140-3, 13/07937-8)

MONTEIRO JUNIOR, JULIO CESAR; KRUGER, ARNE; PALMISANO, GIUSEPPE; WRENGER, CARSTEN. Transporter-Mediated Solutes Uptake as Drug Target in Plasmodium falciparum. FRONTIERS IN PHARMACOLOGY, v. 13, p. 8-pg., 2022-02-07. (15/26722-8, 18/15549-1, 18/18257-1, 18/08820-0, 20/04923-0)

SANTIAGO, VERONICA FEIJOLI; DOMBROWSKI, JAMILLE GREGORIO; KAWAHARA, REBECA; ROSA-FERNANDES, LIVIA; MULE, SIMON NGAO; MURILLO, OSCAR; SANTANA, THAIS VIGGIANI; PACCINI COUTINHO, JOAO VICTOR; MACEDO-DA-SILVA, JANAINA; LAZARI, LUCAS CARDOSO; et al. Complement System Activation Is a Plasma Biomarker Signature during Malaria in Pregnancy. GENES, v. 14, n. 8, p. 20-pg., 2023-08-01. (21/14751-4, 18/15549-1, 20/06747-4, 20/04923-0, 17/04032-5, 21/00140-3, 19/12068-5, 18/20468-0)