Laser-induced fluorescence imaging method to monitor citrus greening disease

Author(s)

Verbi Pereira, Fabiola Manhas ^[1] ; Bastos Pereira Milori, Debora Marcondes ^[1] ; Pereira-Filho, Edenir Rodrigues ^[2] ; Venancio, Andre Leonardo ^{[1, 3]} ; Tavares Russo, Mariana de Sa ^[1] ; do Brasil Cardinali, Marcelo Camponez ^{[4, 1]} ; Martins, Polyana Kelly ^[5] ; Freitas-Astua, Juliana ^{[5, 6]}

Affiliation

^[1] Embrapa Instrumentacao Agr, BR-13561206 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Depto Quim, GAIA, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Depto Fis & Matemat, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[5] Ctr APTA Citros Sylvia Moreira, BR-13490970 Cordeiropolis, SP - Brazil

^[6] Embrapa Mandioca & Fruticultura Trop, BR-44380000 Cruz Das Almas, BA - Brazil

• Document type: Journal article
• Source: COMPUTERS AND ELECTRONICS IN AGRICULTURE; v. 79, n. 1, p. 90-93, OCT 2011.
• Web of Science Citations: 23

Abstract

The potential use of laser-induced fluorescence imaging to monitor citrus greening disease in sweet orange (Citrus sinensis) plants was investigated. For this purpose, an experiment using healthy plants as the control and plants of the same variety inoculated with Candidatus Liberibacter asiaticus (CLas) was performed to verify the fluorescence response through 8 months after bacterial inoculation. A system using laser-induced fluorescence at 473 nm of wavelength excitation was configured. Color descriptors from the collected fluorescence images were determined using the average values for red (R); green (G); blue (B); hue (H); saturation (5); value (V, or Intensity); relative red (rR); relative green (rG); relative blue (rB) and luminosity (L). The data were analyzed using a paired Student's t-test by means of comparison between diseased and healthy plants. The results demonstrate a potential method to identify citrus greening at an early stage of the disease (first month), prior to the onset of visual symptoms at confidence level of 95%. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 07/08618-2 - Use of fluorescence images, laser-induced breakdown spectroscopy and chemometrics strategies to Greening disease study in citrus plantation
• Grantee: Fabiola Manhas Verbi Pereira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral