Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization of Hepatozoon sp in cats from Sao Luis Island, Maranhao, Northeastern Brazil

Full text
Author(s):
de Bortoli, Caroline P. [1] ; Andre, Marcos R. [1] ; Braga, Maria do Socorro C. [1, 2] ; Machado, Rosangela Zacarias [1, 3]
Total Authors: 4
Affiliation:
[1] Univ Estadual Paulista, UNESP, Jaboticabal, SP - Brazil
[2] Univ Estadual Maranhao, Sao Luis, MA - Brazil
[3] Univ Estadual Paulista, Lab Imunoparasitol, Dept Patol Vet, Fac Ciencias Agr & Vet Julio Mesquita Filho, BR-14884900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Parasitology Research; v. 109, n. 4, p. 1189-1192, OCT 2011.
Web of Science Citations: 9
Abstract

Few molecular studies have been done concerning the molecular characterization of Hepatozoon species among domestic and wild felids. The present work aimed to characterize molecularly the presence of Hepatozoon sp. DNA in cat blood samples from Sao Luis Island, Maranhao state, Northeastern Brazil. EDTA-whole blood samples were collected from 200 domestic cats with outdoor and wood areas access from Sao Luis, Maranhao, Brazil. Each sample of extracted DNA was used as a template in PCR reactions aiming to amplify a partial sequence of 18S rRNA of Hepatozoon spp. We also performed sequence alignment to establish the identity of the parasite species infecting these animals using DNA sequences based on 18S rRNA. From 200 sampled cats, Hepatozoon DNA was only found in one animal (0.5%). The found Hepatozoon DNA showed 97% of identity with Hemobartonella felis isolates 1 and 2 from Spain. When analyzing the phylogenetic tree, the found Hepatozoon DNA was in the same clade than H. felis isolates. Our findings suggest that more than one species of Hepatozoon could infect felids in Brazil. (AU)