| Full text | |
| Author(s): |
Mansano, Naira da Silva
;
Vieira, Henrique Rodrigues
;
Araujo-Lopes, Roberta
;
Szawka, Raphael Escorsim
;
Donato Jr, Jose
;
Frazao, Renata
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Endocrinology; v. 164, n. 11, p. 14-pg., 2023-09-23. |
| Abstract | |
It is well-established that the hypothalamic-pituitary-gonadal (HPG) axis is suppressed due to negative energy balance. However, less information is available on whether kisspeptin neuronal activity contributes to fasting-induced responses. In the present study, female and male mice were fasted for 24 hours or provided food ad libitum (fed group) to determine whether acute fasting is sufficient to modulate kisspeptin neuronal activity. In female mice, fasting attenuated luteinizing hormone (LH) and prolactin (PRL) serum levels and increased follicle-stimulating hormone levels compared with the fed group. In contrast, fasting did not affect gonadotropin or PRL secretion in male mice. By measuring genes related to LH pulse generation in micropunches obtained from the arcuate nucleus of the hypothalamus (ARH), we observed that fasting reduced Kiss1 mRNA levels in female and male mice. In contrast, Pdyn expression was upregulated only in fasted female mice, whereas no changes in the Tac2 mRNA levels were observed in both sexes. Interestingly, the frequency and amplitude of the GABAergic postsynaptic currents recorded from ARH kisspeptin neurons (ARHKisspeptin) were reduced in 24-hour fasted female mice but not in males. Additionally, neuropeptide Y induced a hyperpolarization in the resting membrane potential of ARHKisspeptin neurons of fed female mice but not in males. Thus, the response of ARHKisspeptin neurons to fasting is sexually dependent with a female bias, associated with changes in gonadotropins and PRL secretion. Our findings suggest that GABAergic transmission to ARHKisspeptin neurons modulates the activity of the HPG axis during situations of negative energy balance. (AU) | |
| FAPESP's process: | 17/22189-9 - Does the factors that change food intake modulate the activity of Kiss1 neurons? |
| Grantee: | Naira da Silva Mansano |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 19/21707-1 - Food deprivation effects in kisspeptin neurons excitability |
| Grantee: | Renata Frazão |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 20/01318-8 - Central nervous system as a target of growth hormone for the regulation of multiple biological functions |
| Grantee: | Jose Donato Junior |
| Support Opportunities: | Research Projects - Thematic Grants |