Analysis of human biological samples using porous graphitic carbon columns and liquid chromatography-mass spectrometry: a review

Author(s)

Rodrigues, Tais Betoni ; Cunha, Ricardo Leal ; Barci, Paulo Emilio Pereira ; Santos-Neto, alvaro Jose ; Lancas, Fernando Mauro

• Document type: Journal article
• Source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 416, n. 24, p. 21-pg., 2024-08-19.

Abstract

Liquid chromatography-mass spectrometry (LC-MS) has emerged as a powerful analytical technique for analyzing complex biological samples. Among various chromatographic stationary phases, porous graphitic carbon (PGC) columns have attracted significant attention due to their unique properties-such as the ability to separate both polar and non-polar compounds and their stability through all pH ranges and to high temperatures-besides the compatibility with LC-MS. This review discusses the applicability of PGC for SPE and separation in LC-MS-based analyses of human biological samples, highlighting the diverse applications of PGC-LC-MS in analyzing endogenous metabolites, pharmaceuticals, and biomarkers, such as glycans, proteins, oligosaccharides, sugar phosphates, and nucleotides. Additionally, the fundamental principles underlying PGC column chemistry and its advantages, challenges, and advances in method development are explored. This comprehensive review aims to provide researchers and practitioners with a valuable resource for understanding the capabilities and limitations of PGC columns in LC-MS-based analysis of human biological samples, thereby facilitating advancements in analytical methodologies and biomedical research. (AU)

• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 23/07159-7 - Synthesis, characterization and applications of novel graphene-derived sorbents in separation techniques
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/07258-2 - Studies to increase robustness, versatility and efficacy for the determination of alanine, carnosine, catecholamines, caffeine and their metabolites in blood and muscle by LC-MS/MS
• Grantee: Taís Betoni Rodrigues
• Support Opportunities: Scholarships in Brazil - Post-Doctoral